CD3 gamma Antibody (7J2L5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16327

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16327-100ul
NBP3-16327-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7J2L5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD3 gamma Antibodies »

Product Summary for CD3 gamma Antibody (7J2L5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 83-182 of human CD3 gamma (P09693). GMYQCKGSQNKSKPLQVYYRMCQNCIELNAATISGFLFAEIVSIFVLAVGVYFIAGQDGVRQSRASDKQTLLPNDQLYQPLKDREDDQYSHLQGNQLRRN

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD3 gamma Antibody (7J2L5)

Western Blot: CD3 gamma Antibody (7J2L5) [NBP3-16327]

Western Blot: CD3 gamma Antibody (7J2L5) [NBP3-16327]

Western Blot: CD3 gamma Antibody (7J2L5) [NBP3-16327] - Western blot analysis of extracts of various cell lines, using CD3 gamma Rabbit mAb (NBP3-16327) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.
Immunocytochemistry/ Immunofluorescence: CD3 gamma Antibody (7J2L5) [NBP3-16327]

Immunocytochemistry/ Immunofluorescence: CD3 gamma Antibody (7J2L5) [NBP3-16327]

Immunocytochemistry/Immunofluorescence: CD3 gamma Antibody (7J2L5) [NBP3-16327] - Immunofluorescence analysis of Jurkat cells using CD3 gamma Rabbit mAb (NBP3-16327) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Flow Cytometry: CD3 gamma Antibody (7J2L5) [NBP3-16327]

Flow Cytometry: CD3 gamma Antibody (7J2L5) [NBP3-16327]

Flow Cytometry: CD3 gamma Antibody (7J2L5) [NBP3-16327] - Flow cytometry: Jurkat cells were stained with Rabbit IgG isotype control (AC042, 10 ug/ml, blue line) or CD3 gamma Rabbit mAb (NBP3-16327, 2.5 ug/ml orange line), followed by goat anti-Rabbit pAb FITC (1:200 dilution) staining. Non-fluorescently stained Jurkat cells was used as blank control (red line) .
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Applications for CD3 gamma Antibody (7J2L5)

Application
Recommended Usage

Flow Cytometry

1:50 - 1:200

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD3 gamma

CD3G is encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.

Alternate Names

CD3g, IMD17, T3G

Gene Symbol

CD3G

Additional CD3 gamma Products

Product Documents for CD3 gamma Antibody (7J2L5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD3 gamma Antibody (7J2L5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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