CD3 zeta Antibody (06)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20221

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 06

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD3 zeta/CD247 Antibodies »

Product Summary for CD3 zeta Antibody (06)

Immunogen

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD3 zeta (Uniprot#: P20963-1; Arg52-Arg164).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.

Scientific Data Images for CD3 zeta Antibody (06)

CD3 zeta Antibody (06) Flow Cytometry: CD3 zeta Antibody (06) [NBP3-20221]

Flow Cytometry: CD3 zeta Antibody (06) [NBP3-20221]

Flow cytometric analysis of CD3 zeta expression on human whole blood lymphocytes. The cells were treated, stained with NBP3-20221, then a FITC-conjugated secondary antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.

Applications for CD3 zeta Antibody (06)

Application
Recommended Usage

Flow Cytometry

1:25-1:100
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 zeta/CD247

CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C. CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Loss of the zeta chain results in the synthesis of unstable TCRs. A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases.

Long Name

T-cell Receptor zeta

Alternate Names

CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain

Gene Symbol

CD247

Additional CD3 zeta/CD247 Products

Product Documents for CD3 zeta Antibody (06)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD3 zeta Antibody (06)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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