CD3 zeta Antibody (4A12-F6)

Novus Biologicals, part of Bio-Techne | Catalog # H00000919-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4A12-F6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CD3 zeta/CD247 Antibodies »

Product Specifications

Immunogen

CD3Z (AAH25703, 1 a.a. ~ 164 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKWKALFTAAILQAQLPITEAQSFGLLDPKLCYLLDGILFIYGVILTALFLRVKFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPQRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALPPR

Specificity

CD3Z - CD3Z antigen, zeta polypeptide (TiT3 complex)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CD3 zeta Antibody (4A12-F6)

Western Blot: CD3 zeta Antibody (4A12-F6) [H00000919-M01]

Western Blot: CD3 zeta Antibody (4A12-F6) [H00000919-M01]

Western Blot: CD3 zeta Antibody (4A12-F6) [H00000919-M01] - Analysis of CD247 expression in transfected 293T cell line by CD3Z monoclonal antibody (M01), clone 4A12-F6.Lane 1: CD247 transfected lysate (Predicted MW: 18.7 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: CD3 zeta Antibody (4A12-F6) [H00000919-M01]

Immunohistochemistry-Paraffin: CD3 zeta Antibody (4A12-F6) [H00000919-M01]

Immunohistochemistry-Paraffin: CD3 zeta Antibody (4A12-F6) [H00000919-M01] - Analysis of monoclonal antibody to CD3Z on formalin-fixed paraffin-embedded human lymph node tissue. Antibody concentration 5 ug/ml.
Western Blot: CD3 zeta Antibody (4A12-F6) [H00000919-M01]

Western Blot: CD3 zeta Antibody (4A12-F6) [H00000919-M01]

Western Blot: CD3 zeta Antibody (4A12-F6) [H00000919-M01] - CD3Z monoclonal antibody (M01), clone 4A12-F6 Analysis of CD3Z expression in Jurkat.
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Applications for CD3 zeta Antibody (4A12-F6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CD3 zeta/CD247

CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C. CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Loss of the zeta chain results in the synthesis of unstable TCRs. A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases.

Long Name

T-cell Receptor zeta

Alternate Names

CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain

Entrez Gene IDs

919 (Human)

Gene Symbol

CD247

OMIM

186780 (Human)

UniProt

AAH25703 (Human)

Additional CD3 zeta/CD247 Products

Product Documents for CD3 zeta Antibody (4A12-F6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD3 zeta Antibody (4A12-F6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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