I have recently ordered CD3 zeta antibody (4B10) Catalog number: NBP1-42561 Lot: 4310 . According to the datasheet the concentration should be labeled on the vial. Unfortunately, I missed to find any concentrational detail on the vial, neither the concentration nor the amount. It would be grateful if you let me informed about the concentration.

The formulation for this product is ascites fluid, therefore the concentration is unknown as it is not purified antibody. There would be many other protein factors contained in the ascitic fluid making determination of an exact antibody concentration impossible without additional purification. The reason an isotype is known and provided is due to the fact that this is a monoclonal antibody and not because additional purifications were performed for that isotype.

I would like to stain for human CD3 and IL12Rb2 antigen in human skin sections. Skin is PFA fixed and paraffin embedded. Will CD3 Ab (NBP1-42561) work on the sections after antigen retrieval?

NBP1-42561 has been validated in IHC-Pariffin sections for human tissue. As long as the target is expressed in your tissue, this antibody will work for detection. We generally recommend the Citrate buffer pH 6.0 antigen retrieval.

What is the concentration of Cat: NBP1-42561?

This antibody is unpurified and supplied as ascitic fluid containing 0.03% sodium azide. The concentration was not determined empirically since it unpurified, but the estimate is between 5-10 mg/ml.

CD3 zeta Antibody (4B10) - BSA Free

Name: CD3 zeta Antibody (4B10) - BSA Free
Brand: Novus Biologicals
SKU: NBP1-42561

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42561

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Product Datasheet / COA / SDS

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 4B10

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all CD3 zeta/CD247 Antibodies »

Product Summary for CD3 zeta Antibody (4B10) - BSA Free

Immunogen

Purified recombinant fragment of human CD3 zeta expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD3 zeta Antibody (4B10) - BSA Free

Western Blot: CD3 zeta Antibody (4B10)BSA Free [NBP1-42561]

Western Blot: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis using CD247 mAb against CD247(AA: 52-164)-hIgGFc transfected HEK293 cell lysate.

Immunocytochemistry/ Immunofluorescence: CD3 zeta Antibody (4B10) - BSA Free [NBP1-42561]

Immunocytochemistry/Immunofluorescence: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of K562 cells using anti-CD247 mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry-Paraffin: CD3 zeta Antibody (4B10) - BSA Free [NBP1-42561]

Immunohistochemistry-Paraffin: CD3 zeta Antibody (4B10) [NBP1-42561] - Analysis of human Thymus tissues using anti-CD247 mouse mAb

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Applications for CD3 zeta Antibody (4B10) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 zeta/CD247

CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.

Long Name

T-cell Receptor zeta

Alternate Names

CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain

Entrez Gene IDs

919 (Human)

Gene Symbol

CD247

UniProt

P20963 (Human)

Additional CD3 zeta/CD247 Products

Product Documents for CD3 zeta Antibody (4B10) - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CD3 zeta Antibody (4B10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD3 zeta Antibody (4B10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42561

Key Product Details

Species Reactivity

Validated:

Applications

Label

Antibody Source

Format

Concentration

View all CD3 zeta/CD247 Antibodies »

Product Summary for CD3 zeta Antibody (4B10) - BSA Free

Immunogen

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for CD3 zeta Antibody (4B10) - BSA Free

Western Blot: CD3 zeta Antibody (4B10)BSA Free [NBP1-42561]

Immunocytochemistry/ Immunofluorescence: CD3 zeta Antibody (4B10) - BSA Free [NBP1-42561]

Immunohistochemistry-Paraffin: CD3 zeta Antibody (4B10) - BSA Free [NBP1-42561]

Flow Cytometry: CD3 zeta Antibody (4B10) - BSA Free [NBP1-42561]

Applications for CD3 zeta Antibody (4B10) - BSA Free

ELISA

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: CD3 zeta/CD247

Long Name

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional CD3 zeta/CD247 Products

Product Documents for CD3 zeta Antibody (4B10) - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CD3 zeta Antibody (4B10) - BSA Free