CD3 zeta Antibody (H146-968) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-44683

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

CyTOF-ready, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Armenian Hamster IgG2 kappa Clone # H146-968

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD3 zeta/CD247 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acids 151-164 of mouse CD3 zeta.

Specificity

The Armenian hamster antibody H146-968 reacts with CD3 zeta chain (CD247), which is a component of TCR/CD3 complex expressed on T cells.

Clonality

Monoclonal

Host

Armenian Hamster

Isotype

IgG2 kappa

Scientific Data Images for CD3 zeta Antibody (H146-968) - Azide and BSA Free

Western Blot: CD3 zeta Antibody (H146-968)Azide and BSA Free [NBP1-44683]

Western Blot: CD3 zeta Antibody (H146-968)Azide and BSA Free [NBP1-44683]

Western Blot: CD3 zeta Antibody (H146-968) - Azide Free [NBP1-44683] - Analysis of CD3 zeta (CD247) in lysate of murine splenocytes (reducing conditions) by the monoclonal antibody H146-968.
Flow Cytometry: CD3 zeta Antibody (H146-968) - Azide and BSA Free [NBP1-44683]

Flow Cytometry: CD3 zeta Antibody (H146-968) - Azide and BSA Free [NBP1-44683]

Flow Cytometry: CD3 zeta Antibody (H146-968) - Azide Free [NBP1-44683] - Analysis (intracellular staining) of CD3 zeta (CD247) in human peripheral blood using the monoclonal antibody H146-968 PE
Flow Cytometry: CD3 zeta Antibody (H146-968) - Azide and BSA Free [NBP1-44683]

Flow Cytometry: CD3 zeta Antibody (H146-968) - Azide and BSA Free [NBP1-44683]

Flow Cytometry: CD3 zeta Antibody (H146-968) - Azide Free [NBP1-44683] - Flow Cytometry: CD3 zeta Antibody (H146-968) [PE] [NBP1-44683PE] - A cell surface stain was performed on hPBMCs with CD3 Zeta antibody (H146-968) NBP1-44683 (top image) and a matched isotype control (bottom image). Cells were incubated in an antibody dilution of 1:200 for 20 minutes at room temperature. Both antibodies were conjugated to PE.

Applications for CD3 zeta Antibody (H146-968) - Azide and BSA Free

Application
Recommended Usage

Flow (Intracellular)

1-5 ug/ml

Flow Cytometry

1-5 ug/ml

Immunocytochemistry/ Immunofluorescence

4 ug/ml

Immunoprecipitation

1:50

Western Blot

1-2 ug/ml
Application Notes
Western blotting: reducing conditions; positive control: MOLT-4 cells, murine splenocytes. This antibody is Cytof ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 0.2 um filter sterilized

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD3 zeta/CD247

CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.

Long Name

T-cell Receptor zeta

Alternate Names

CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain

Gene Symbol

CD247

Additional CD3 zeta/CD247 Products

Product Documents for CD3 zeta Antibody (H146-968) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD3 zeta Antibody (H146-968) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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