CD3 zeta Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85750

Novus Biologicals, part of Bio-Techne
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NBP1-85750
NBP1-85750-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all CD3 zeta/CD247 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KFSRSADAPAYQQGQNQLYNELNLGRREEYDVLDKRRGRDPEMGGKPRRKNPQEGLYNELQKDKMAEAYSEIGMKGERRRGKGHDGLYQGLSTATKDTYDALHMQALP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (89%). Reactivity reported in scientific literature (PMID: 23977094)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD3 zeta Antibody

Immunohistochemistry-Paraffin: CD3 zeta Antibody [NBP1-85750]

Immunohistochemistry-Paraffin: CD3 zeta Antibody [NBP1-85750]

Immunohistochemistry-Paraffin: CD3 zeta Antibody [NBP1-85750] - Analysis in human lymph node and pancreas tissues using NBP1-85750 antibody. Corresponding CD247 RNA-seq data are presented for the same tissues.
Western Blot: CD3 zeta Antibody [NBP1-85750]

Western Blot: CD3 zeta Antibody [NBP1-85750]

Western Blot: CD3 zeta Antibody [NBP1-85750] - Analysis in human cell line MOLT-4.
Immunohistochemistry-Paraffin: CD3 zeta Antibody [NBP1-85750]

Immunohistochemistry-Paraffin: CD3 zeta Antibody [NBP1-85750]

Immunohistochemistry-Paraffin: CD3 zeta Antibody [NBP1-85750] - Staining of human Pancreas shows no positivity in exocrine glandular cells as expected.
View 4 more images

Applications for CD3 zeta Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 zeta/CD247

CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C. CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Loss of the zeta chain results in the synthesis of unstable TCRs. A decrease of CD3 zeta has been described in T cells from patients with cancer, lupus and chronic infectious diseases.

Long Name

T-cell Receptor zeta

Alternate Names

CD247, CD3H, CD3Q, CD3Z, IMD25, T3Z, TCR Zeta Chain

Gene Symbol

CD247

Additional CD3 zeta/CD247 Products

Product Documents for CD3 zeta Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD3 zeta Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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