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CD30 Ligand/TNFSF8 Antibody (RM153) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11992CL1

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, Flow Cytometry, Functional Assay

Label

CoraFluor 1

Antibody Source

Recombinant Monoclonal Rat IgG2b Kappa Clone # RM153

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was raised by immunising Sprague Dawley rats with the CHO cells stably transfected with murine CD30 Ligand/TNFSF8 and fusing the splenocytes with P3U1 myeloma cells.

Specificity

This antibody is specific for murine CD30 Ligand/TNFSF8, a 40-kDa type II transmembrane protein member of the TNF superfamily. Interaction of CD30 with CD30 Ligand/TNFSF8 plays an important role in T cell activation/costimulation.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2b Kappa

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for CD30 Ligand/TNFSF8 Antibody (RM153) [CoraFluor™ 1]

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional Assay

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CD30 Ligand/TNFSF8

CD153 is a 39-40 kD type II membrane glycoprotein, also known as CD30 ligand or CD30L. This TNF superfamily member is expressed on activated T cells. CD153 has been reported to stimulate B-cell proliferation, antigen-specific antibody production, polyclonal immunoglobulin secretion, and T cell activation and proliferation. The RM153 antibody inhibits binding of CD153 to CD30.

Alternate Names

CD153, CD30L, TNFSF8

Gene Symbol

TNFSF8

Additional CD30 Ligand/TNFSF8 Products

Product Documents for CD30 Ligand/TNFSF8 Antibody (RM153) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD30 Ligand/TNFSF8 Antibody (RM153) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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