CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70374

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1C6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CD30/TNFRSF8 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 19-379 of human TNFRSF8 (NP_001234) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

61.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free

Western Blot: CD30/TNFRSF8 Antibody (OTI1C6)Azide and BSA Free [NBP2-70374]

Western Blot: CD30/TNFRSF8 Antibody (OTI1C6)Azide and BSA Free [NBP2-70374]

Western Blot: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNFRSF8.
Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374]

Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374]

Immunohistochemistry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of Human prostate tissue.
Flow Cytometry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374]

Flow Cytometry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374]

Flow Cytometry: CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free [NBP2-70374] - Analysis of K562 and Ramos cells, using anti-TNFRSF8 antibody (TA801630), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue) (1:100).
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Applications for CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD30/TNFRSF8

CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins. (1,2) Murine CD30 is expressed predominantly in the thymus, and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A. (1) In anti-CD3epsilon-activated spleen cells, CD30 is expressed primarily on the surface of CD8+ T cells with peak expression on days 4 and 5. Stimulation of CD30+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN; production. (1) While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.

Alternate Names

CD30, TNFRSF8

Gene Symbol

TNFRSF8

Additional CD30/TNFRSF8 Products

Product Documents for CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD30/TNFRSF8 Antibody (OTI1C6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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