CD300a/LMIR1 Antibody (MEM-260) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-58726
![Flow Cytometry: CD300a/LMIR1 Antibody (MEM-260) - BSA Free [NB110-58726]](https://resources.bio-techne.com/images/products/CD300a-LMIR1-Antibody-MEM-260-Flow-Cytometry-NB110-58726-img0002.jpg "Flow Cytometry: CD300a/LMIR1 Antibody (MEM-260) - BSA Free [NB110-58726]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # MEM-260
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
HPB acute lymphoid leukemia cell line (NM_007261.2).
Specificity
This antibody (clone MEM-260) reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CD300a/LMIR1 Antibody (MEM-260) - BSA Free
Flow Cytometry: CD300a/LMIR1 Antibody (MEM-260) - BSA Free [NB110-58726]
Flow Cytometry: CD300a/LMIR1 Antibody (MEM-260) [NB110-58726] - staining of human peripheral blood cells with anti-CD300a (MEM-260) purified, GAM-APC.![Flow Cytometry: CD300a/LMIR1 Antibody (MEM-260) - BSA Free [NB110-58726]](https://resources.bio-techne.com/images/products/101876_1.jpg "Flow Cytometry: CD300a/LMIR1 Antibody (MEM-260) - BSA Free [NB110-58726]")
Flow Cytometry: CD300a/LMIR1 Antibody (MEM-260) - BSA Free [NB110-58726]
Flow Cytometry: CD300a/LMIR1 Antibody (MEM-260) [NB110-58726] - Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) PE.Applications for CD300a/LMIR1 Antibody (MEM-260) - BSA Free
Application
Recommended Usage
Flow Cytometry
2 ug/ml
Western Blot
1:1000
Application Notes
Western Blot: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. This antibody is CyTOF ready.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CD300a/LMIR1
Alternate Names
CD300a, CLM8, CMRF-35H, IGSF12, IRC1, IRC2, IRp60, LMIR1, MAIR-I, Mcipr1, NKRL, Pigr4
Entrez Gene IDs
11314 (Human)
Gene Symbol
CD300A
UniProt
Additional CD300a/LMIR1 Products
Product Documents for CD300a/LMIR1 Antibody (MEM-260) - BSA Free
Product Specific Notices for CD300a/LMIR1 Antibody (MEM-260) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...