This antibody (clone MEM-260) reacts with CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for CD300a/LMIR1 Antibody (MEM-260) [PE/Atto594]

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CD300a/LMIR1

Human CD300a, otherwise known as IRp60 (inhibitory receptor protein 60), is a 60kDa transmembrane glycoprotein expressed by natural killer cells (NK), T and B cell subsets, monocytes, neutrophils, macrophages, granulocytes, dendritic cells (DC) and mast cells. Studies have shown that the cross-linking of CD300a on NK results in a down-regulation of their cytolytic activity, whilst cross-linking of CD300a on mast cells, inhibits IgE-induced degranulation and SCF-mediated survival. Furthermore, cross-linking of CD300a on the surface of eosinophils has been shown to significantly inhibit their survival, chemotaxis and activation in response to certain inflammatory cytokines, whilst eosinophil-derived MBP (major basic protein) and EDN (neurotoxin), can down-regulate CD300a expression on mast cells in vitro.

Alternate Names

CD300a, CLM8, CMRF-35H, IGSF12, IRC1, IRC2, IRp60, LMIR1, MAIR-I, Mcipr1, NKRL, Pigr4

Gene Symbol

CD300A

Additional CD300a/LMIR1 Products

Product Documents for CD300a/LMIR1 Antibody (MEM-260) [PE/Atto594]

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CD300a/LMIR1 Antibody (MEM-260) [PE/Atto594]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

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Flow Cytometry

CD300a/LMIR1 Antibody (MEM-260) [PE/Atto594]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58726PEATT594

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all CD300a/LMIR1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Applications for CD300a/LMIR1 Antibody (MEM-260) [PE/Atto594]

Flow Cytometry

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: CD300a/LMIR1

Alternate Names

Gene Symbol

Additional CD300a/LMIR1 Products

Product Documents for CD300a/LMIR1 Antibody (MEM-260) [PE/Atto594]

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CD300a/LMIR1 Antibody (MEM-260) [PE/Atto594]

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