CD300LG/Nepmucin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00118

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD300g/Nepmucin Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human CD300LG/Nepmucin (Accession#: AAH25395.1; Met1-Arg247)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD300LG/Nepmucin Antibody

Western Blot: CD300LG/Nepmucin Antibody [NBP3-00118]

Western Blot: CD300LG/Nepmucin Antibody [NBP3-00118]

Western Blot: CD300LG/Nepmucin Antibody [NBP3-00118] - Anti-CD300LG/Nepmucin rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 45 kDa
Immunoprecipitation: CD300LG/Nepmucin Antibody [NBP3-00118]

Immunoprecipitation: CD300LG/Nepmucin Antibody [NBP3-00118]

Immunoprecipitation: CD300LG/Nepmucin Antibody [NBP3-00118] - CD300LG/Nepmucin was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. 0.5 ul anti-CD300LG/Nepmucin rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CD300LG/Nepmucin rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 38 kDa. Observed band size: 38 kDa

Applications for CD300LG/Nepmucin Antibody

Application
Recommended Usage

Immunoprecipitation

1-2 uL/mg of lysate

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD300g/Nepmucin

Members of the CD300 (see MIM 606786)-like (CD300L) family, such as CD300LG, are widely expressed on hematopoieticcells. All CD300L proteins are type I cell surface glycoproteins that contain a single immunoglobulin (Ig) V-likedomain (Takatsu et al., 2006 (PubMed 16876123)).(supplied by OMIM)

Alternate Names

CD300LG, CLM9, Nepmucin, TREM-4

Gene Symbol

CD300LG

Additional CD300g/Nepmucin Products

Product Documents for CD300LG/Nepmucin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD300LG/Nepmucin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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