CD31/PECAM-1 Antibody (BLR127H)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14710

Recombinant Monoclonal
Novus Biologicals, part of Bio-Techne
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NBP3-14710
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit Clone # BLR127H

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD31/PECAM-1 Antibodies »

Product Specifications

Immunogen

residues 28-601 (ECD)

Clonality

Monoclonal

Host

Rabbit

Scientific Data Images for CD31/PECAM-1 Antibody (BLR127H)

Western Blot: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710]

Western Blot: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710]

Western Blot: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710] - Whole cell lysate (10 ug) from MOLT-4, HEK293T, U2OS, Jurkat, A-549, GaMG, MCF-7, and HUVEC-C cells prepared using NETN lysis buffer. Rabbit anti-CD31/PECAM-1 recombinant monoclonal antibody used at 1:1000. HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710]

Immunohistochemistry: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710]

Immunohistochemistry: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710] - Rabbit anti- CD31/PECAM-1 recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.
CD31/PECAM-1 Antibody (BLR127H)

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710] -

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (BLR127H) [NBP3-14710] -: Rabbit antiCD31/PECAM-1 recombinant monoclonal antibody[BLR127H]. Secondary: HRP-conjugated goat anti-rabbit IgG.Substrate: DAB.

Applications for CD31/PECAM-1 Antibody (BLR127H)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 to 1:500

Immunohistochemistry

1:100 to 1:500

Immunohistochemistry-Paraffin

1:100 to 1:500

Immunoprecipitation

6 µl/1 mg lysate

Western Blot

1:1,000
Application Notes
0. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.

Formulation, Preparation, and Storage

Purification

>95%

Formulation

Borate Buffered Saline (BBS) pH 8.2 with , BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CD31/PECAM-1

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for CD31/PECAM-1 Antibody (BLR127H)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD31/PECAM-1 Antibody (BLR127H)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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