CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33154

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # C31.3

Format

Azide and BSA Free

Concentration

1.0 mg/ml

View all CD31/PECAM-1 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human CD31/PECAM-1 protein (Uniprot: P16284)

Reactivity Notes

Weakly reacts with Rat.

Localization

Cell surface and cytoplasm of endothelial cells

Specificity

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 monoclonal antibody reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Marker

Endothelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-15202).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free

Western Blot: CD31/PECAM-1 Antibody (C31.3)Azide and BSA Free [NBP2-33154]

Western Blot: CD31/PECAM-1 Antibody (C31.3)Azide and BSA Free [NBP2-33154]

Western Blot: CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free [NBP2-33154] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free.
Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free [NBP2-33154]

Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free [NBP2-33154]

Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free [NBP2-33154] - Flow Cytometry of human CD31 on Jurkat cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled CD31/PECAM-1 Antibody (C31.7) - Azide and BSA Free.
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free [NBP2-33154]

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free [NBP2-33154]

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free [NBP2-33154] - Formalin-paraffin human Angiosarcoma stained with CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free.
View 3 more images

Applications for CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

0.5-1ug/ml

Immunohistochemistry-Frozen

0.5-1.0ug/ml

Immunohistochemistry-Paraffin

0.5-1.0ug/ml

Western Blot

0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD31/PECAM-1

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD31/PECAM-1 Antibody (C31.3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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