Key Product Details

Species Reactivity

Validated:

Sheep

Cited:

Bovine, Equine, Ovine

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation

Cited:

Flow Cytometry, IF/IHC, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CO.3E1D4

Concentration

1.0 mg/ml

View all CD31/PECAM-1 Antibodies »

Product Specifications

Immunogen

Ovine leucocytes

Reactivity Notes

Predicted cross-reactivities: Goat, Bovine

Specificity

NB100-65900 is specific for ovine CD31, also known as PECAM-1. Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes. CD31 is also highly expressed by ovine endothelial cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

82.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD31/PECAM-1 Antibody (CO.3E1D4)

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (CO.3E-1D4) - BSA Free [NB100-65900]

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (CO.3E-1D4) - BSA Free [NB100-65900]

Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Valvular endothelial cell (VEC) markers. Sections of the sheep aortic valve leaflet stained with antibodies against the endothelial cell markers CD31/PECAM-1, CD34, VE-Cadherin (CD144), or von Willebrand Factor (vWF). Represented are the aortic wall and a section of the valvular leaflet (near the free edge), with "a" and "v" indicating the aortic and ventricular sides of the leaflet, respectively. Scale bars, 100 um. Image collected and cropped by CiteAb from the following publication (www.frontiersin.org/article/10.3389/fcvm.2018.00105/full) licensed under a CC-BY license.
Flow Cytometry: CD31/PECAM-1 Antibody (CO.3E-1D4) - BSA Free [NB100-65900]

Flow Cytometry: CD31/PECAM-1 Antibody (CO.3E-1D4) - BSA Free [NB100-65900]

Flow Cytometry: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Staining of sheep peripheral blood platelets with Mouse anti Bovine CD31/PECAM-1 Antibody (CO.3E-1D4).
Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (CO.3E-1D4) - BSA Free [NB100-65900]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (CO.3E-1D4) - BSA Free [NB100-65900]

Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (CO.3E-1D4) [NB100-65900] - Bovine Lung stained with CD31 (green) and DAPI counterstain. Image provided courtesy of Hala Nijmeh, PhD and the PI, Evgenia V. Gerasimovskaya, PhD. Section of Critical Care Medicine, Department of Pediatrics, University of Colorado Anschutz Medical Center.

Applications for CD31/PECAM-1 Antibody (CO.3E1D4)

Application
Recommended Usage

Flow Cytometry

1:10-1:25

Immunohistochemistry

1:50

Immunohistochemistry-Frozen

1:50

Immunoprecipitation

1:10-1:500
Application Notes
Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 30159315).
Clone CO.3E1D4 is reported to inhibit homotypic leucocyte aggregation induced by anti CD43 antibodies. Removal of sodium azide is recommended prior to use in fuctional assays.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD31/PECAM-1

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

Long Name

Platelet Endothelial Cell Adhesion Molecule 1

Alternate Names

CD31, EndoCAM, PECA1, PECAM-1, PECAM1

Gene Symbol

PECAM1

Additional CD31/PECAM-1 Products

Product Documents for CD31/PECAM-1 Antibody (CO.3E1D4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD31/PECAM-1 Antibody (CO.3E1D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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