CD31/PECAM-1 Antibody (GT0010) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13523
Key Product Details
Species Reactivity
Mouse
Applications
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Armenian Hamster IgG Clone # GT0010
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The immunogen used to generate this antibody corresponds to human CD31/PECAM-1
Clonality
Monoclonal
Host
Armenian Hamster
Isotype
IgG
Theoretical MW
83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Centrifuge briefly prior to opening.
Scientific Data Images for CD31/PECAM-1 Antibody (GT0010) - Azide and BSA Free
![Flow Cytometry: CD31/PECAM-1 Antibody (GT0010) [NBP3-13523]](https://resources.bio-techne.com/images/products/CD31-PECAM-1-Antibody-GT0010-Flow-Cytometry-NBP3-13523-img0003.jpg "Flow Cytometry: CD31/PECAM-1 Antibody (GT0010) [NBP3-13523]")
Flow Cytometry: CD31/PECAM-1 Antibody (GT0010) [NBP3-13523]
Flow Cytometry: CD31/PECAM-1 Antibody (GT0010) [NBP3-13523] - CD31/PECAM-1 antibody detects CD31/PECAM-1 protein by flow cytometry analysis. Sample: Raw264.7 cell fixed in 4% paraformaldehyde at 4C for 5 min. Black: Isotype control dilution: 1:100. Green: CD31/PECAM-1 antibody (NBP3-13523) dilution: 1:100.Applications for CD31/PECAM-1 Antibody (GT0010) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD31/PECAM-1
PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.
References
1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial
2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3
3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9
Long Name
Platelet Endothelial Cell Adhesion Molecule 1
Alternate Names
CD31, EndoCAM, PECA1, PECAM-1, PECAM1
Gene Symbol
PECAM1
Additional CD31/PECAM-1 Products
Product Documents for CD31/PECAM-1 Antibody (GT0010) - Azide and BSA Free
Product Specific Notices for CD31/PECAM-1 Antibody (GT0010) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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