CD34 Antibody (MEC 14.7) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80642
![Immunocytochemistry/ Immunofluorescence: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]](https://resources.bio-techne.com/images/products/CD34-Antibody-MEC-14-7-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80642-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]")
![Immunocytochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]](https://resources.bio-techne.com/images/products/CD34-Antibody-MEC-14-7-Azide-and-BSA-Free-Immunocytochemistry-NBP2-80642-img0007.jpg "Immunocytochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse, Human (Negative)
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # MEC 14.7
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for CD34 Antibody (MEC 14.7) - Azide and BSA Free
Immunogen
Murine transformed endothelioma cell line t-end.
Reactivity Notes
This antibody does not detect Human CD34 and is an excellent tool for marking host derived endothelial cells/vasculature in human cancer xenografts on mouse.
Localization
Membrane; Single-pass type I membrane protein.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for CD34 Antibody (MEC 14.7) - Azide and BSA Free
Immunocytochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]
Immunocytochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642] - Analysis of a human renal cancer tissue section using CD34 antibody (clone MEC 14.7) at 1:100 dilution. The antibody did not detect human CD34 which is an expected outcome for this clone. This section was included in NB600-1071's IHC validation testing as![Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]](https://resources.bio-techne.com/images/products/CD34-Antibody-MEC-14-7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80642-img0008.jpg "Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]")
Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]
Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642] - Analysis of CD34 on human renal cancer xenograft using CD34 antibody (clone MEC 14.7). The antibody detected the endothelial cells in the tumor vasculature which are originating from the host (mouse). Image from the standard format of this antibody.![Flow Cytometry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]](https://resources.bio-techne.com/images/products/CD34-Antibody-MEC-14-7-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80642-img0009.jpg "Flow Cytometry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]")
Flow Cytometry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]
Flow Cytometry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642] - CD34 (MEC 14.7) antibody was tested at 1:250 in WEHI-3 cells with DyLight 488 (green) alongside a matched isotype control (black). Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]](https://resources.bio-techne.com/images/products/CD34-Antibody-MEC-14-7-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80642-img0002.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]")
![Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]](https://resources.bio-techne.com/images/products/CD34-Antibody-MEC-14-7-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80642-img0003.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]")
![Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]](https://resources.bio-techne.com/images/products/CD34-Antibody-MEC-14-7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80642-img0004.jpg "Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]")
![Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]](https://resources.bio-techne.com/images/products/CD34-Antibody-MEC-14-7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80642-img0005.jpg "Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]")
![Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]](https://resources.bio-techne.com/images/products/CD34-Antibody-MEC-14-7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80642-img0006.jpg "Immunohistochemistry: CD34 Antibody (MEC 14.7) - Azide and BSA Free [NBP2-80642]")
Applications for CD34 Antibody (MEC 14.7) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100-1:2000
Flow Cytometry
1 ug per million cells
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100 - 1:250
Immunohistochemistry-Frozen
1:100 - 1:250
Immunohistochemistry-Paraffin
1:100 - 1:250
Immunoprecipitation
1:10-1:500
Western Blot
1:250
Application Notes
This CD34 (MEC 14.7) antibody is useful for Immunohistochemistry (on both paraffin-embedded and frozen sections), Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western blot, Immunoprecipitation and ELISA. Antigen retrieval is required for IHC-Paraffin.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Tris-Glycine, 0.15 M NaCl
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD34
CD34 has commonly been used as a marker for the diagnosis and classification of various diseases and pathologies including leukemia and solitary fibrous tumor (SFT) (2,5). In terms of immunohistochemistry and histopathology, CD34 has been the most common marker for SFT and is expressed in ~79% of cases (5). In addition to its use as a cell marker, CD34-postive (CD34+) hematopoietic stem cells have been used therapeutically in patients following radiation or chemotherapy due to their regenerative potential (6). There are several clinical trials showing promising results for CD34+ cell therapy for cardiovascular diseases including heart failure, ischemia, dilated cardiomyopathy, acute myocardial infarction, and angina (6). Besides hematopoietic lineages, CD34 is also expressed in non-hematopoietic cells including mesenchymal stem cells, endothelial cells and progenitors, fibrocytes, muscle satellite cells, and some cancer stem cells (1,3). While the clinical and cell therapy applications of CD34 as a cell marker is well documented, the function of CD34 is less understood but has been implicated in many cellular processes such as adhesion, proliferation, signal transduction, differentiation, and progenitor phenotype maintenance (1,3).
References
1. Sidney, L. E., Branch, M. J., Dunphy, S. E., Dua, H. S., & Hopkinson, A. (2014). Concise review: evidence for CD34 as a common marker for diverse progenitors. Stem cells (Dayton, Ohio), 32(6), 1380-1389. https://doi.org/10.1002/stem.1661
2. Krause, D. S., Fackler, M. J., Civin, C. I., & May, W. S. (1996). CD34: structure, biology, and clinical utility. Blood, 87(1), 1-13
3. Kapoor, S., Shenoy, S. P., & Bose, B. (2020). CD34 cells in somatic, regenerative and cancer stem cells: Developmental biology, cell therapy, and omics big data perspective. Journal of cellular biochemistry, 121(5-6), 3058-3069. https://doi.org/10.1002/jcb.29571
4. Uniprot (P28906)
5. Davanzo, B., Emerson, R. E., Lisy, M., Koniaris, L. G., & Kays, J. K. (2018). Solitary fibrous tumor. Translational gastroenterology and hepatology, 3, 94. https://doi.org/10.21037/tgh.2018.11.02
6. Prasad, M., Corban, M. T., Henry, T. D., Dietz, A. B., Lerman, L. O., & Lerman, A. (2020). Promise of autologous CD34+ stem/progenitor cell therapy for treatment of cardiovascular disease. Cardiovascular research, 116(8), 1424-1433. https://doi.org/10.1093/cvr/cvaa027
Alternate Names
CD34, HPCA1
Gene Symbol
CD34
Additional CD34 Products
Product Documents for CD34 Antibody (MEC 14.7) - Azide and BSA Free
Product Specific Notices for CD34 Antibody (MEC 14.7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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