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CD35 Antibody (rCR1/8597) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24139

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-24139

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # rCR1/8597

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant fragment (around aa600-900) of human CD35 protein (exact sequence is proprietary)

Localization

Cell surface.

Specificity

Anti-CD35 is considered a mature B-cell marker which labels follicular dendritic reticulum cells and tumors derived from such cells such as follicular dendritic cell tumor/sarcoma.

Marker

Follicular Dendritic Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD35 Antibody (rCR1/8597) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD35 Antibody (rCR1/8597) - Azide and BSA Free [NBP3-24139] -

Formalin-fixed, paraffin-embedded human tonsil stained with CD35 antibody (rCR1/8597) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.

Applications for CD35 Antibody (rCR1/8597) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD35

CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

Alternate Names

C3BR, CD35, CR1, KN

Gene Symbol

CR1

Additional CD35 Products

Product Documents for CD35 Antibody (rCR1/8597) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD35 Antibody (rCR1/8597) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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