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CD37 Antibody (WR17) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81048

Recombinant Monoclonal Antibody. Clone WR17 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP2-81048-0.025mg
NBP2-81048-0.2mg

Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # WR17

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for CD37 Antibody (WR17) - Chimeric - Azide and BSA Free

Immunogen

Chronic lymphocytic leukaemia cells.

Specificity

Recognises human CD37.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD37 Antibody (WR17) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD37 Antibody (WR17) - Chimeric - Azide and BSA Free [NBP2-81048]

Immunocytochemistry/ Immunofluorescence: CD37 Antibody (WR17) - Chimeric - Azide and BSA Free [NBP2-81048]

Immunocytochemistry/Immunofluorescence: CD37 Antibody (WR17) [NBP2-81048] - Immunofluorescence staining of fixed Daudi cells with anti-CD37 antibody (WR17) [NBP2-81048]. Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of WR17 [NBP2-81048] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81048, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

Applications for CD37 Antibody (WR17) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques. This antibody binds CD37, staining, amongst others, B-cells and B-cell tumours.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD37

CD37, also known as GP52-40, TSPAN26, MGC120234. Enterz Protein NP_001035120. It is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It may play a role in T-cell-B-cell interactions. Alternate splicing results in multiple transcript variants encoding different isoforms.

Alternate Names

CD37, TSPAN26

Gene Symbol

CD37

Additional CD37 Products

Product Documents for CD37 Antibody (WR17) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD37 Antibody (WR17) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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