CD4 Antibody (13B8.2) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52670
Recombinant Monoclonal Antibody. Clone 13B8.2 was used by HLDA to establish CD designation.
![Flow Cytometry: CD4 Antibody (13B8.2) - Chimeric - Azide and BSA Free [NBP2-52670] Flow Cytometry: CD4 Antibody (13B8.2) - Chimeric - Azide and BSA Free [NBP2-52670]](https://resources.bio-techne.com/images/products/CD4-Antibody-13B8-2-Chimeric-Flow-Cytometry-NBP2-52670-img0001.jpg)
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 13B8.2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Human HSB2-T cells.
Specificity
Recognises human CD4.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images for CD4 Antibody (13B8.2) - Chimeric - Azide and BSA Free
Flow Cytometry: CD4 Antibody (13B8.2) - Chimeric - Azide and BSA Free [NBP2-52670]
Flow Cytometry: CD4 Antibody (13B8.2) - Chimeric [NBP2-52670] - Flow-cytometry using anti-CD4 antibody 13B8.2. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of 13B8.2 (NBP2-52670, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.Applications for CD4 Antibody (13B8.2) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Flow Cytometry
1:10 - 1:1000
Immunohistochemistry-Frozen
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD4
Given its critical role in T cell development, CD4 also has diverse immunology-related functions. CD4 acts as a coreceptor with the T-cell receptor (TCR) during T cell activation and thymic differentiation by binding directly to major histocompatibility complex (MHC) class II antigens and associating with the protein tyrosine kinase, Lck (4). This interaction contributes to the formation of the immunological synapse (5). Defects in antigen presentation cause dysfunction of CD4+ T cells and the almost complete loss of MHC II expression on B cells in peripheral blood, as observed in severe combined immunodeficiency (SCID) (6). CD4 also functions as a receptor for the human immunodeficiency virus (HIV) by binding to gp120, the envelope glycoprotein of HIV-1. It has been shown that the V-like domains are critical for binding to gp120 (7). In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage (8).
References
1. Omri, B., Crisanti, P., Alliot, F., Marty, M., Rutin, J., Levallois, C., . . . Pessac, B. (1994). CD4 expression in neurons of the central nervous system. International Immunology, 6(3), 377-385. doi:10.1093/intimm/6.3.377
2. Wan, Y. Y., & Flavell, R. A. (2009). How diverse-CD4 effector T cells and their functions. Journal of Molecular Cell Biology, 1(1), 20-36. doi:10.1093/jmcb/mjp001
3. Wu, H., Myszka, D. G., Tendian, S. W., Brouillette, C. G., Sweet, R. W., Chaiken, I. M., & Hendrickson, W. A. (1996). Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding. Proceedings of the National Academy of Sciences, 93(26), 15030-15035. doi:10.1073/pnas.93.26.15030
4. Doyle, C., & Strominger, J. L. (1987). Interaction between CD4 and class II MHC molecules mediates cell adhesion. Nature, 330, 256-259. doi:10.1038/330256a0
5. Vignali, D. A. (2010). CD4 on the road to coreceptor status. The Journal of Immunology, 184(11), 5933-5934. doi:10.4049/jimmunol.1090037
6. Tasher, D., & Dalal, I. (2012). The genetic basis of severe combined immunodeficiency and its variants. The Application of Clinical Genetics, 5, 67-80. doi:10.2147/tacg.s18693
7. Arthos, J., Deen, K. C., Chaikin, M. A., Fornwald, J. A., Sathe, G., Sattentau, Q. J., . . . Sweet, R. W. (1989). Identification of the residues in human CD4 critical for the binding of HIV. Cell, 57(3), 469-481. doi:10.1016/0092-8674(89)90922-7
8. Buttini, M., Westland, C. E., Masliah, E., Yafeh, A. M., Wyss-Coray, T., Mucke, L. (1998). Novel role of human cd4 molecule identified in neurodegeneration. Nature Medicine, 4(4), 441-446. doi:10.1038/nm0498-441
Alternate Names
CD4
Gene Symbol
CD4
Additional CD4 Products
Product Documents for CD4 Antibody (13B8.2) - Chimeric - Azide and BSA Free
Product Specific Notices for CD4 Antibody (13B8.2) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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