CD4 Antibody (BL-155-1C11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76423

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-76423
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Multiplex Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # BL-155-1C11

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD4 Antibodies »

Product Specifications

Immunogen

Recombinant protein representing the extracellular domain of human CD4

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD4 Antibody (BL-155-1C11) - BSA Free

Immunoprecipitation: CD4 Antibody (BL-155-1C11) [NBP2-76423]

Immunoprecipitation: CD4 Antibody (BL-155-1C11) [NBP2-76423]

Immunoprecipitation: CD4 Antibody (BL-155-1C11) [NBP2-76423] - Detection of human CD4 by WB of immunoprecipitates from MOLT-4 lysate. Antibodies: Rabbit anti-CD4 recombinant monoclonal [BL-155-1C11] (NBP2-76423) and another rabbit anti-CD4 antibody.
CD4 Antibody (BL-155-1C11)

Western Blot: CD4 Antibody (BL-155-1C11) [NBP2-76423] -

Western Blot: CD4 Antibody (BL-155-1C11) [NBP2-76423] - Whole cell lysate (10 ug) from U-937, HEK293T, MOLT-4, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD4 recombinant monoclonal antibody [BL-155-1C11] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].
CD4 Antibody (BL-155-1C11)

Flow Cytometry- CD4 Antibody (BL-155-1C11) [NBP2-76423] -

Flow Cytometry- CD4 Antibody (BL-155-1C11) [NBP2-76423] -Rabbit anti-CD4 recombinantmonoclonal [BL-155-1C11] or isotype control (unshaded). Secondary: DyLight® 488-conjugated goatanti-rabbit IgG.
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Applications for CD4 Antibody (BL-155-1C11) - BSA Free

Application
Recommended Usage

Flow Cytometry

Live or cells fixed in 4% formaldehyde and permeabilized with 90%

Immunohistochemistry

1:100 - 1:500

Immunoprecipitation

20 ul/mg lysate

Multiplex Immunofluorescence

1:250

Western Blot

1:1000
Application Notes
Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Borate Buffered Saline (BBS) pH 8.2

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 2 - 8 C / 1 year from date of receipt

Background: CD4

CD4 (cluster of differentiation 4), also known as L3T4 or T4, is a 55 kDa single chain type I transmembrane glycoprotein belonging to the immunoglobin (Ig) superfamily. CD4 is predominantly expressed on most thymocytes, a subset of mature T lymphocytes, and weakly on monocytes, tissue macrophages, dendritic cells, and granulocytes. It is also expressed on neurons and glial cells in the brain (1). CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells (T helper cells) further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (2). The extracellular region of CD4 consists of 372 amino acids (aa) with four immunoglobin-like domains (D1-D4). The structures of D1 and D3 resemble variable (IgV) domains while D2 and D4 resemble constant (IgC) domains (3).

Given its critical role in T cell development, CD4 also has diverse immunology-related functions. CD4 acts as a coreceptor with the T-cell receptor (TCR) during T cell activation and thymic differentiation by binding directly to major histocompatibility complex (MHC) class II antigens and associating with the protein tyrosine kinase, Lck (4). This interaction contributes to the formation of the immunological synapse (5). Defects in antigen presentation cause dysfunction of CD4+ T cells and the almost complete loss of MHC II expression on B cells in peripheral blood, as observed in severe combined immunodeficiency (SCID) (6). CD4 also functions as a receptor for the human immunodeficiency virus (HIV) by binding to gp120, the envelope glycoprotein of HIV-1. It has been shown that the V-like domains are critical for binding to gp120 (7). In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage (8).

References

1. Omri, B., Crisanti, P., Alliot, F., Marty, M., Rutin, J., Levallois, C., . . . Pessac, B. (1994). CD4 expression in neurons of the central nervous system. International Immunology, 6(3), 377-385. doi:10.1093/intimm/6.3.377

2. Wan, Y. Y., & Flavell, R. A. (2009). How diverse-CD4 effector T cells and their functions. Journal of Molecular Cell Biology, 1(1), 20-36. doi:10.1093/jmcb/mjp001

3. Wu, H., Myszka, D. G., Tendian, S. W., Brouillette, C. G., Sweet, R. W., Chaiken, I. M., & Hendrickson, W. A. (1996). Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding. Proceedings of the National Academy of Sciences, 93(26), 15030-15035. doi:10.1073/pnas.93.26.15030

4. Doyle, C., & Strominger, J. L. (1987). Interaction between CD4 and class II MHC molecules mediates cell adhesion. Nature, 330, 256-259. doi:10.1038/330256a0

5. Vignali, D. A. (2010). CD4 on the road to coreceptor status. The Journal of Immunology, 184(11), 5933-5934. doi:10.4049/jimmunol.1090037

6. Tasher, D., & Dalal, I. (2012). The genetic basis of severe combined immunodeficiency and its variants. The Application of Clinical Genetics, 5, 67-80. doi:10.2147/tacg.s18693

7. Arthos, J., Deen, K. C., Chaikin, M. A., Fornwald, J. A., Sathe, G., Sattentau, Q. J., . . . Sweet, R. W. (1989). Identification of the residues in human CD4 critical for the binding of HIV. Cell, 57(3), 469-481. doi:10.1016/0092-8674(89)90922-7

8. Buttini, M., Westland, C. E., Masliah, E., Yafeh, A. M., Wyss-Coray, T., Mucke, L. (1998). Novel role of human cd4 molecule identified in neurodegeneration. Nature Medicine, 4(4), 441-446. doi:10.1038/nm0498-441

Alternate Names

CD4

Gene Symbol

CD4

Additional CD4 Products

Product Documents for CD4 Antibody (BL-155-1C11) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD4 Antibody (BL-155-1C11) - BSA Free

This Product sold under License from Bethyl Laboratories, Inc.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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