CD4 Antibody (BLR167J)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-14741
Recombinant Monoclonal
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR167J
Concentration
1 mg/ml
Product Specifications
Immunogen
between 407 and 457 (C-term)
Clonality
Monoclonal
Host
Rabbit
Scientific Data Images for CD4 Antibody (BLR167J)
Western Blot: CD4 Antibody (BLR167J) [NBP3-14741]
Western Blot: CD4 Antibody (BLR167J) [NBP3-14741] - Whole cell lysate (10 ug) from NIH 3T3, BW5147.3, mouseThymus, 70Z/3, and EL4 cells prepared using NETN lysisbuffer. Antibody: Rabbit anti-CD4 recombinant monoclonalantibody [BLR167J] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgGChemiluminescence with an exposure timeof 30 seconds. Lower Panel: Rabbit anti-Actin recombinantmonoclonal antibody [BLR057F].Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741]
Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse lung, : Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741]
Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse spleen, Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.Applications for CD4 Antibody (BLR167J)
Application
Recommended Usage
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Immunoprecipitation
6 ul/1 mg lysate
Western Blot
1:1000
Application Notes
For Immunohistochemistry, epitope retrieval with Tris-EDTA pH9.0 is recommended for Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with , BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: CD4
Given its critical role in T cell development, CD4 also has diverse immunology-related functions. CD4 acts as a coreceptor with the T-cell receptor (TCR) during T cell activation and thymic differentiation by binding directly to major histocompatibility complex (MHC) class II antigens and associating with the protein tyrosine kinase, Lck (4). This interaction contributes to the formation of the immunological synapse (5). Defects in antigen presentation cause dysfunction of CD4+ T cells and the almost complete loss of MHC II expression on B cells in peripheral blood, as observed in severe combined immunodeficiency (SCID) (6). CD4 also functions as a receptor for the human immunodeficiency virus (HIV) by binding to gp120, the envelope glycoprotein of HIV-1. It has been shown that the V-like domains are critical for binding to gp120 (7). In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage (8).
References
1. Omri, B., Crisanti, P., Alliot, F., Marty, M., Rutin, J., Levallois, C., . . . Pessac, B. (1994). CD4 expression in neurons of the central nervous system. International Immunology, 6(3), 377-385. doi:10.1093/intimm/6.3.377
2. Wan, Y. Y., & Flavell, R. A. (2009). How diverse-CD4 effector T cells and their functions. Journal of Molecular Cell Biology, 1(1), 20-36. doi:10.1093/jmcb/mjp001
3. Wu, H., Myszka, D. G., Tendian, S. W., Brouillette, C. G., Sweet, R. W., Chaiken, I. M., & Hendrickson, W. A. (1996). Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding. Proceedings of the National Academy of Sciences, 93(26), 15030-15035. doi:10.1073/pnas.93.26.15030
4. Doyle, C., & Strominger, J. L. (1987). Interaction between CD4 and class II MHC molecules mediates cell adhesion. Nature, 330, 256-259. doi:10.1038/330256a0
5. Vignali, D. A. (2010). CD4 on the road to coreceptor status. The Journal of Immunology, 184(11), 5933-5934. doi:10.4049/jimmunol.1090037
6. Tasher, D., & Dalal, I. (2012). The genetic basis of severe combined immunodeficiency and its variants. The Application of Clinical Genetics, 5, 67-80. doi:10.2147/tacg.s18693
7. Arthos, J., Deen, K. C., Chaikin, M. A., Fornwald, J. A., Sathe, G., Sattentau, Q. J., . . . Sweet, R. W. (1989). Identification of the residues in human CD4 critical for the binding of HIV. Cell, 57(3), 469-481. doi:10.1016/0092-8674(89)90922-7
8. Buttini, M., Westland, C. E., Masliah, E., Yafeh, A. M., Wyss-Coray, T., Mucke, L. (1998). Novel role of human cd4 molecule identified in neurodegeneration. Nature Medicine, 4(4), 441-446. doi:10.1038/nm0498-441
Alternate Names
CD4
Gene Symbol
CD4
Additional CD4 Products
Product Documents for CD4 Antibody (BLR167J)
Product Specific Notices for CD4 Antibody (BLR167J)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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