CD4 Antibody (CE9.1 (Clenoliximab)) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52681

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-52681-0.025mg
NBP2-52681-0.2mg
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Key Product Details

Species Reactivity

Human, Chimpanzee

Applications

Block/Neutralize, CyTOF-ready, ELISA, Flow Cytometry, Immunohistochemistry, Surface Plasmon Resonance, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Lambda Clone # CE9.1 (Clenoliximab)

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD4 Antibodies »

Product Specifications

Immunogen

Domain 1 of human CD4.

Specificity

The antibody binds to human CD4 with a Kd of 1.0 nM and an ED50 of 0.2 ug/ml.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Lambda

Scientific Data Images for CD4 Antibody (CE9.1 (Clenoliximab)) - Chimeric - Azide and BSA Free

Flow Cytometry: CD4 Antibody (CE9.1 (Clenoliximab)) - Chimeric - Azide and BSA Free [NBP2-52681]

Flow Cytometry: CD4 Antibody (CE9.1 (Clenoliximab)) - Chimeric - Azide and BSA Free [NBP2-52681]

Flow Cytometry: CD4 Antibody (CE9.1 (Clenoliximab)) - Chimeric [NBP2-52681] - Flow-cytometry using the anti-CD4 research biosimilar antibody Clenoliximab (NBP2-52681) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Clenoliximab (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
Western Blot: CD4 Antibody (CE9.1 (Clenoliximab))ChimericAzide and BSA Free [NBP2-52681]

Western Blot: CD4 Antibody (CE9.1 (Clenoliximab))ChimericAzide and BSA Free [NBP2-52681]

Western Blot: CD4 Antibody (CE9.1 (Clenoliximab)) - Chimeric [NBP2-52681] - Human thyroid tumour samples were resolved on a 15% SDS PAGE gel and blots probed with the human IgG4-chimeric version of CE9.1 (NBP2-75910) at 1.5 ug/ml before detection using an anti-human IgG4 secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD4 is 51kDa (arrowhead). Here some non-specific bands can also be seen. NBP2-75910 successfully detected CD4 in human thyroid tissue samples.
Immunohistochemistry: CD4 Antibody (CE9.1 (Clenoliximab)) - Chimeric - Azide and BSA Free [NBP2-52681]

Immunohistochemistry: CD4 Antibody (CE9.1 (Clenoliximab)) - Chimeric - Azide and BSA Free [NBP2-52681]

Immunohistochemistry: CD4 Antibody (CE9.1 (Clenoliximab)) - Chimeric [NBP2-52681] - Anti-CD4 staining of formaldehyde fixed paraffin embedded guinea pig skin tissue, at 40x magnification. The human IgG4-chimeric version of CE9.1 (Clenoliximab) (NBP2-75910) was used to stain samples at a concentration of 3 ug/ml.

Applications for CD4 Antibody (CE9.1 (Clenoliximab)) - Chimeric - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000Blocking/Neutralizing 50 - 200 molar excess

Immunohistochemistry

1:10 - 1:500
Application Notes
This antibody is Cytof ready. NOT FOR THERAPEUTIC USE - This is a research-grade biosimilar. This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 (primatized) format, for improved compatibility with existing reagents, assays and techniques. The antibody binds specifically to CD4, a molecule which is part of the immunolgobulin super-family, expressed on the surface of helper T cells. The CD4 molecule acts as a co-receptor in the activation of T cells, aiding the interaction between the TCR and MHC II of the antigen-presenting cell. The antibody is classified as "primatized", as the variable regions of the light and heavy chains are obtained from primates, while the lamda light chain and gamma-4 heavy chain constant domains are human. The antibody was engineered from the primatized IgG1 monoclonal antibody Keliximab by substituting two key residues. The role of the antibody as an immunosuppressor has been tested in vivo, by intravenous administration in chimpanzees and human volunteers. The antibody reached Phase II clinical trials with Biogen-Idec.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD4

CD4 (cluster of differentiation 4), also known as L3T4 or T4, is a 55 kDa single chain type I transmembrane glycoprotein belonging to the immunoglobin (Ig) superfamily. CD4 is predominantly expressed on most thymocytes, a subset of mature T lymphocytes, and weakly on monocytes, tissue macrophages, dendritic cells, and granulocytes. It is also expressed on neurons and glial cells in the brain (1). CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells (T helper cells) further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (2). The extracellular region of CD4 consists of 372 amino acids (aa) with four immunoglobin-like domains (D1-D4). The structures of D1 and D3 resemble variable (IgV) domains while D2 and D4 resemble constant (IgC) domains (3).

Given its critical role in T cell development, CD4 also has diverse immunology-related functions. CD4 acts as a coreceptor with the T-cell receptor (TCR) during T cell activation and thymic differentiation by binding directly to major histocompatibility complex (MHC) class II antigens and associating with the protein tyrosine kinase, Lck (4). This interaction contributes to the formation of the immunological synapse (5). Defects in antigen presentation cause dysfunction of CD4+ T cells and the almost complete loss of MHC II expression on B cells in peripheral blood, as observed in severe combined immunodeficiency (SCID) (6). CD4 also functions as a receptor for the human immunodeficiency virus (HIV) by binding to gp120, the envelope glycoprotein of HIV-1. It has been shown that the V-like domains are critical for binding to gp120 (7). In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage (8).

References

1. Omri, B., Crisanti, P., Alliot, F., Marty, M., Rutin, J., Levallois, C., . . . Pessac, B. (1994). CD4 expression in neurons of the central nervous system. International Immunology, 6(3), 377-385. doi:10.1093/intimm/6.3.377

2. Wan, Y. Y., & Flavell, R. A. (2009). How diverse-CD4 effector T cells and their functions. Journal of Molecular Cell Biology, 1(1), 20-36. doi:10.1093/jmcb/mjp001

3. Wu, H., Myszka, D. G., Tendian, S. W., Brouillette, C. G., Sweet, R. W., Chaiken, I. M., & Hendrickson, W. A. (1996). Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding. Proceedings of the National Academy of Sciences, 93(26), 15030-15035. doi:10.1073/pnas.93.26.15030

4. Doyle, C., & Strominger, J. L. (1987). Interaction between CD4 and class II MHC molecules mediates cell adhesion. Nature, 330, 256-259. doi:10.1038/330256a0

5. Vignali, D. A. (2010). CD4 on the road to coreceptor status. The Journal of Immunology, 184(11), 5933-5934. doi:10.4049/jimmunol.1090037

6. Tasher, D., & Dalal, I. (2012). The genetic basis of severe combined immunodeficiency and its variants. The Application of Clinical Genetics, 5, 67-80. doi:10.2147/tacg.s18693

7. Arthos, J., Deen, K. C., Chaikin, M. A., Fornwald, J. A., Sathe, G., Sattentau, Q. J., . . . Sweet, R. W. (1989). Identification of the residues in human CD4 critical for the binding of HIV. Cell, 57(3), 469-481. doi:10.1016/0092-8674(89)90922-7

8. Buttini, M., Westland, C. E., Masliah, E., Yafeh, A. M., Wyss-Coray, T., Mucke, L. (1998). Novel role of human cd4 molecule identified in neurodegeneration. Nature Medicine, 4(4), 441-446. doi:10.1038/nm0498-441

Alternate Names

CD4, biosimilar antibody, biosimilars

Gene Symbol

CD4

Additional CD4 Products

Product Documents for CD4 Antibody (CE9.1 (Clenoliximab)) - Chimeric - Azide and BSA Free

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Product Specific Notices for CD4 Antibody (CE9.1 (Clenoliximab)) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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