CD4 Antibody (GK1.5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-26585
![Immunohistochemistry-Frozen: CD4 Antibody (GK1.5) - Azide and BSA Free [NBP2-26585]](https://resources.bio-techne.com/images/products/CD4-Antibody-GK1-5-Azide-Free-Immunohistochemistry-Frozen-NBP2-26585-img0008.jpg "Immunohistochemistry-Frozen: CD4 Antibody (GK1.5) - Azide and BSA Free [NBP2-26585]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Cell depletion, CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Inhibition Activity
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG Kappa Clone # GK1.5
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The mouse cytotoxic T lymphocyte line V4 was used as the immunogen for the CD4 antibody.
Clonality
Monoclonal
Host
Rat
Isotype
IgG Kappa
Scientific Data Images for CD4 Antibody (GK1.5) - Azide and BSA Free
Immunohistochemistry-Frozen: CD4 Antibody (GK1.5) - Azide and BSA Free [NBP2-26585]
Immunohistochemistry-Frozen: CD4 Antibody (GK1.5) - Azide Free [NBP2-26585] - CD4 was detected in immersion fixed frozen sections of mouse intestine using Rat Anti-Mouse CD4 Monoclonal Antibody (Clone GK1.5) at 1 ug/mL overnight at 4 degrees C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes of lymphocytes.
Flow (Cell Surface): CD4 Antibody (GK1.5) [Azide Free] [NBP2-26585] - Analysis using the FITC conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of NBP2-26584. Green represents isotype control (20212C); red represents mouse CD4 NBP2-26584 antibody.
Flow (Cell Surface): CD4 Antibody (GK1.5) [Azide Free] [NBP2-26585] - Analysis using the Allophycocyanin conjugate of NBP2-26585. Staining of CD4 in 10^6 BALB/c mouse splenocytes using 1 ug of this antibody. Green represents isotype control; red represents mouse CD4 this antibody.
Applications for CD4 Antibody (GK1.5) - Azide and BSA Free
Application
Recommended Usage
Flow (Cell Surface)
2.5 ug / 1 million cells
Flow Cytometry
2.5 ug / 1 million cells
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1-25 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
Filter sterilized PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CD4
Given its critical role in T cell development, CD4 also has diverse immunology-related functions. CD4 acts as a coreceptor with the T-cell receptor (TCR) during T cell activation and thymic differentiation by binding directly to major histocompatibility complex (MHC) class II antigens and associating with the protein tyrosine kinase, Lck (4). This interaction contributes to the formation of the immunological synapse (5). Defects in antigen presentation cause dysfunction of CD4+ T cells and the almost complete loss of MHC II expression on B cells in peripheral blood, as observed in severe combined immunodeficiency (SCID) (6). CD4 also functions as a receptor for the human immunodeficiency virus (HIV) by binding to gp120, the envelope glycoprotein of HIV-1. It has been shown that the V-like domains are critical for binding to gp120 (7). In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage (8).
References
1. Omri, B., Crisanti, P., Alliot, F., Marty, M., Rutin, J., Levallois, C., . . . Pessac, B. (1994). CD4 expression in neurons of the central nervous system. International Immunology, 6(3), 377-385. doi:10.1093/intimm/6.3.377
2. Wan, Y. Y., & Flavell, R. A. (2009). How diverse-CD4 effector T cells and their functions. Journal of Molecular Cell Biology, 1(1), 20-36. doi:10.1093/jmcb/mjp001
3. Wu, H., Myszka, D. G., Tendian, S. W., Brouillette, C. G., Sweet, R. W., Chaiken, I. M., & Hendrickson, W. A. (1996). Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding. Proceedings of the National Academy of Sciences, 93(26), 15030-15035. doi:10.1073/pnas.93.26.15030
4. Doyle, C., & Strominger, J. L. (1987). Interaction between CD4 and class II MHC molecules mediates cell adhesion. Nature, 330, 256-259. doi:10.1038/330256a0
5. Vignali, D. A. (2010). CD4 on the road to coreceptor status. The Journal of Immunology, 184(11), 5933-5934. doi:10.4049/jimmunol.1090037
6. Tasher, D., & Dalal, I. (2012). The genetic basis of severe combined immunodeficiency and its variants. The Application of Clinical Genetics, 5, 67-80. doi:10.2147/tacg.s18693
7. Arthos, J., Deen, K. C., Chaikin, M. A., Fornwald, J. A., Sathe, G., Sattentau, Q. J., . . . Sweet, R. W. (1989). Identification of the residues in human CD4 critical for the binding of HIV. Cell, 57(3), 469-481. doi:10.1016/0092-8674(89)90922-7
8. Buttini, M., Westland, C. E., Masliah, E., Yafeh, A. M., Wyss-Coray, T., Mucke, L. (1998). Novel role of human cd4 molecule identified in neurodegeneration. Nature Medicine, 4(4), 441-446. doi:10.1038/nm0498-441
Alternate Names
CD4
Gene Symbol
CD4
Additional CD4 Products
Product Documents for CD4 Antibody (GK1.5) - Azide and BSA Free
Product Specific Notices for CD4 Antibody (GK1.5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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