CD4 Antibody (MT310) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52663

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-52663-0.025mg
NBP2-52663-0.2mg
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Key Product Details

Species Reactivity

Human, Cynomolgus Monkey, Rhesus Macaque

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Cited:

IF/ICC

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # MT310

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD4 Antibodies »

Product Specifications

Immunogen

CD4

Specificity

mt310 binds to the extracellular domain D1 of CD4 -the same site as the HIV-1 gp120-binding site

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD4 Antibody (MT310) - Chimeric - Azide and BSA Free

Flow Cytometry: CD4 Antibody (MT310) - Chimeric - Azide and BSA Free [NBP2-52663]

Flow Cytometry: CD4 Antibody (MT310) - Chimeric - Azide and BSA Free [NBP2-52663]

Flow Cytometry: CD4 Antibody (MT310) - Chimeric [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
Flow Cytometry: CD4 Antibody (MT310) - Chimeric - Azide and BSA Free [NBP2-52663]

Flow Cytometry: CD4 Antibody (MT310) - Chimeric - Azide and BSA Free [NBP2-52663]

Flow Cytometry: CD4 Antibody (MT310) - Chimeric [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
Flow Cytometry: CD4 Antibody (MT310) - Chimeric - Azide and BSA Free [NBP2-52663]

Flow Cytometry: CD4 Antibody (MT310) - Chimeric - Azide and BSA Free [NBP2-52663]

Flow Cytometry: CD4 Antibody (MT310) - Chimeric [NBP2-52663] - Flow-cytometry using anti-CD4 antibody MT310 (NBP2-52663) Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of MT310 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Applications for CD4 Antibody (MT310) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques. mt310 can inhibit gp120 binding to CD4, which blocks HIV infectivity of CD4+ T lymphocytes, as well as the binding of MHCII, which results in the block of T-cell activation, possibly leading to the induction of anergy. Antibody binding to CD4 additionally causes the induction of GM-CSF, TNF-alpha (only after 20 h) and IL-10.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD4

CD4 (cluster of differentiation 4), also known as L3T4 or T4, is a 55 kDa single chain type I transmembrane glycoprotein belonging to the immunoglobin (Ig) superfamily. CD4 is predominantly expressed on most thymocytes, a subset of mature T lymphocytes, and weakly on monocytes, tissue macrophages, dendritic cells, and granulocytes. It is also expressed on neurons and glial cells in the brain (1). CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells (T helper cells) further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (2). The extracellular region of CD4 consists of 372 amino acids (aa) with four immunoglobin-like domains (D1-D4). The structures of D1 and D3 resemble variable (IgV) domains while D2 and D4 resemble constant (IgC) domains (3).

Given its critical role in T cell development, CD4 also has diverse immunology-related functions. CD4 acts as a coreceptor with the T-cell receptor (TCR) during T cell activation and thymic differentiation by binding directly to major histocompatibility complex (MHC) class II antigens and associating with the protein tyrosine kinase, Lck (4). This interaction contributes to the formation of the immunological synapse (5). Defects in antigen presentation cause dysfunction of CD4+ T cells and the almost complete loss of MHC II expression on B cells in peripheral blood, as observed in severe combined immunodeficiency (SCID) (6). CD4 also functions as a receptor for the human immunodeficiency virus (HIV) by binding to gp120, the envelope glycoprotein of HIV-1. It has been shown that the V-like domains are critical for binding to gp120 (7). In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage (8).

References

1. Omri, B., Crisanti, P., Alliot, F., Marty, M., Rutin, J., Levallois, C., . . . Pessac, B. (1994). CD4 expression in neurons of the central nervous system. International Immunology, 6(3), 377-385. doi:10.1093/intimm/6.3.377

2. Wan, Y. Y., & Flavell, R. A. (2009). How diverse-CD4 effector T cells and their functions. Journal of Molecular Cell Biology, 1(1), 20-36. doi:10.1093/jmcb/mjp001

3. Wu, H., Myszka, D. G., Tendian, S. W., Brouillette, C. G., Sweet, R. W., Chaiken, I. M., & Hendrickson, W. A. (1996). Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding. Proceedings of the National Academy of Sciences, 93(26), 15030-15035. doi:10.1073/pnas.93.26.15030

4. Doyle, C., & Strominger, J. L. (1987). Interaction between CD4 and class II MHC molecules mediates cell adhesion. Nature, 330, 256-259. doi:10.1038/330256a0

5. Vignali, D. A. (2010). CD4 on the road to coreceptor status. The Journal of Immunology, 184(11), 5933-5934. doi:10.4049/jimmunol.1090037

6. Tasher, D., & Dalal, I. (2012). The genetic basis of severe combined immunodeficiency and its variants. The Application of Clinical Genetics, 5, 67-80. doi:10.2147/tacg.s18693

7. Arthos, J., Deen, K. C., Chaikin, M. A., Fornwald, J. A., Sathe, G., Sattentau, Q. J., . . . Sweet, R. W. (1989). Identification of the residues in human CD4 critical for the binding of HIV. Cell, 57(3), 469-481. doi:10.1016/0092-8674(89)90922-7

8. Buttini, M., Westland, C. E., Masliah, E., Yafeh, A. M., Wyss-Coray, T., Mucke, L. (1998). Novel role of human cd4 molecule identified in neurodegeneration. Nature Medicine, 4(4), 441-446. doi:10.1038/nm0498-441

Alternate Names

CD4, biosimilar antibody, biosimilars

Gene Symbol

CD4

Additional CD4 Products

Product Documents for CD4 Antibody (MT310) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD4 Antibody (MT310) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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