CD4 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18057
![Immunocytochemistry/ Immunofluorescence: CD4 Antibody [NBP3-18057]](https://resources.bio-techne.com/images/products/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18057-img0002.jpg "Immunocytochemistry/ Immunofluorescence: CD4 Antibody [NBP3-18057]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial recombinant protein made to an N-terminal portion of human CD4 (amino acids 26-390) [UniProt P01730]
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CD4 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: CD4 Antibody [NBP3-18057]
Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP3-18057] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD4 Antibody (NBP3-18057) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: CD4 Antibody [NBP3-18057]](https://resources.bio-techne.com/images/products/CD4-Antibody-Immunohistochemistry-Paraffin-NBP3-18057-img0001.jpg "Immunohistochemistry-Paraffin: CD4 Antibody [NBP3-18057]")
Immunohistochemistry-Paraffin: CD4 Antibody [NBP3-18057]
Immunohistochemistry-Paraffin: CD4 Antibody [NBP3-18057] - Analysis of a FFPE tissue section of human spleen using 1:200 dilution of CD4 antibody (NBP3-18057). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.![Flow Cytometry: CD4 Antibody [NBP3-18057]](https://resources.bio-techne.com/images/products/CD4-Antibody-Flow-Cytometry-NBP3-18057-img0003.jpg "Flow Cytometry: CD4 Antibody [NBP3-18057]")
Flow Cytometry: CD4 Antibody [NBP3-18057]
Flow Cytometry: CD4 Antibody [NBP3-18057] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP3-18057 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).Applications for CD4 Antibody - BSA Free
Application
Recommended Usage
Flow (Intracellular)
2 - 3 ug/ml
Flow Cytometry
2 - 3 ug/ml
Immunocytochemistry/ Immunofluorescence
1 - 2 ug/ml
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
1 - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD4
Alternate Names
CD4, biosimilar antibody, biosimilars
Gene Symbol
CD4
Additional CD4 Products
Product Documents for CD4 Antibody - BSA Free
Product Specific Notices for CD4 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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