CD40 Ligand/TNFSF5 Antibody (YMF323.6) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-81114
Recombinant Monoclonal Antibody
![Immunocytochemistry/ Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (YMF323.6) - Chimeric - Azide and BSA Free [NBP2-81114]](https://resources.bio-techne.com/images/products/CD40-Ligand-TNFSF5-Antibody-YMF323-6-Immunocytochemistry-Immunofluorescence-NBP2-81114-img0001.jpg "Immunocytochemistry/ Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (YMF323.6) - Chimeric - Azide and BSA Free [NBP2-81114]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # YMF323.6
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary
Immunogen
CD40 Ligand/TNFSF5
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Scientific Data Images
Immunocytochemistry/ Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (YMF323.6) - Chimeric - Azide and BSA Free [NBP2-81114]
Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (YMF323.6) [NBP2-81114] - Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with [NBP2-81114]. Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of YMF323.6 [NBP2-81114] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81114, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.Applications
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: CD40 Ligand/TNFSF5
Alternate Names
CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP
Gene Symbol
CD40LG
Additional CD40 Ligand/TNFSF5 Products
- All Products for CD40 Ligand/TNFSF5
- CD40 Ligand/TNFSF5 cDNA Clones
- CD40 Ligand/TNFSF5 ELISA Kits
- CD40 Ligand/TNFSF5 Luminex Assays
- CD40 Ligand/TNFSF5 Lysates
- CD40 Ligand/TNFSF5 Primary Antibodies
- CD40 Ligand/TNFSF5 Proteins and Enzymes
- CD40 Ligand/TNFSF5 Simple Plex
- CD40 Ligand/TNFSF5 Small Molecules and Peptides
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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