CD40/TNFRSF5 Antibody (G28.5) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52648

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-52648-0.025mg
NBP2-52648-0.2mg
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Conjugate
Catalog #
Alexa Fluor 350
NBP2-52648AF350
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Alexa Fluor 405
NBP2-52648AF405
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Alexa Fluor 488
NBP2-52648AF488
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Alexa Fluor 532
NBP2-52648AF532
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Alexa Fluor 594
NBP2-52648AF594
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Alexa Fluor 647
NBP2-52648AF647
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Alexa Fluor 700
NBP2-52648AF700
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Alexa Fluor 750
NBP2-52648AF750
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Allophycocyanin
NBP2-52648APC
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Allophycocyanin/Cy7
NBP2-52648APCCY7
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Biotin
NBP2-52648B
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CoraFluor 1
NBP2-52648CL1
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DyLight 350
NBP2-52648UV
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DyLight 405
NBP2-52648V
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DyLight 488
NBP2-52648G
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DyLight 550
NBP2-52648R
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DyLight 594
NBP2-52648DL594
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DyLight 650
NBP2-52648C
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DyLight 680
NBP2-52648FR
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DyLight 755
NBP2-52648IR
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FITC
NBP2-52648F
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HRP
NBP2-52648H
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Janelia Fluor 525
NBP2-52648JF525
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Janelia Fluor 549
NBP2-52648JF549
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Janelia Fluor 585
NBP2-52648JF585
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Janelia Fluor 635
NBP2-52648JF635
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Janelia Fluor 646
NBP2-52648JF646
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Janelia Fluor 669
NBP2-52648JF669
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mFluor Violet 450 SE
NBP2-52648MFV450
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mFluor Violet 500 SE
NBP2-52648MFV500
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mFluor Violet 610 SE
NBP2-52648MFV610
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PE
NBP2-52648PE
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PE/Atto594
NBP2-52648PEATT594
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PE/Cy5.5
NBP2-52648PECY55
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PE/Cy7
NBP2-52648PECY7
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PerCP
NBP2-52648PCP
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Key Product Details

Species Reactivity

Validated:

Human

Applications

CyTOF-ready, Flow Cytometry, Functional, Immunohistochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # G28.5

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD40/TNFRSF5 Antibodies »

Product Summary for CD40/TNFRSF5 Antibody (G28.5) - Chimeric - Azide and BSA Free

Immunogen

Human erythrocyte-rosette-negative tonsillar lymphocytes.

Specificity

48 kD Type I glycoprotein, a member of the TNFR superfamily primarily expressed on normal or malignant B cells or B cell lines, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD40/TNFRSF5 Antibody (G28.5) - Chimeric - Azide and BSA Free

Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric - Azide and BSA Free [NBP2-52648]

Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric - Azide and BSA Free [NBP2-52648]

Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Cynomolgus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric - Azide and BSA Free [NBP2-52648]

Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric - Azide and BSA Free [NBP2-52648]

Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric - Azide and BSA Free [NBP2-52648]

Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric - Azide and BSA Free [NBP2-52648]

Flow Cytometry: CD40/TNFRSF5 Antibody (G28.5) - Chimeric [NBP2-52648] - Flow-cytometry using anti-CD40 antibody G28.5. Rhesus monkey lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of G28.5 (NBP2-52648, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Applications for CD40/TNFRSF5 Antibody (G28.5) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG1 format, for improved compatibility with existing reagents, assays and techniques. This antibody detects CD40/TNFRSF5 by immunostaining. The original mouse IgG1 format was shown to stimular B-cells (Bishop 2012 and references therein).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD40/TNFRSF5

CD40 and its ligand CD154 are members of the tumor necrosis factor receptor (TNFR) and TNF families, respectively, that play key roles in signaling pathways mediating cell growth, survival and differentiation in B-lymphocytes (reviewed in Quezada et al 2004). The CD40 receptor is a 45-50 kDa glycoprotein and is expressed on the surface of B-lymphocytes, some activated T-cells, monocytes, follicular dendritic cells, basal epithelial cells, and in some epithelial and non-epithelial carcinomas. The functions of CD40 have been most extensively studied in B-cells. Ligation of B CD40 by CD154, expressed on activated T cells, stimulates B cell proliferation, differentiation, isotype switching, upregulation of surface molecules contributing to antigen presentation, development of the germinal center, and the humoral memory response. Several distinct structural motifs in the CD40 cytoplasmic domain regaulate various CD40 signaling pathways. A major CD40 signaling pathway activated from CD154 ligand binding is the canonical pathway to the transcription factor family NF-kB, a family of genes mediating immune and inflammatory responses. Although CD40 has been extensively studied as a plasma membrane-associated growth factor membrane receptor. it has also been identified in the cytoplasm and nucleus of normal and neoplastic B-lymphoid cells (Lin-Lee et al. 2006). Other growth factor receptors, including EGF, FGF, and TGF-B have also been identified in the nuclus. It is thought that plasma membrane receptor signaling may be followed by nuclear migration of signaling pathway components. The presence of CD40 in the nucleus of activated normal B lymphocytes and neoplastic B-lymphoid cells suggests that CD40 may play a more complex role in regulating essential growth and survival pathways in B-lymphocytes than previously thought.

Alternate Names

CD40, TNFRSF5

Gene Symbol

CD40

Additional CD40/TNFRSF5 Products

Product Documents for CD40/TNFRSF5 Antibody (G28.5) - Chimeric - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD40/TNFRSF5 Antibody (G28.5) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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