Recognises the human CD42b cell surface antigen, also known as platelet glycoprotein GP1B. CD42b is expressed by platelets and megakaryocytes. Clone AK2 has been reported to block the binding of von Willebrand Factor (VWF) to platelets (1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD42b/GPIb alpha Antibody (AK2)

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Analysis using the FITC conjugate of NB100-63790. Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD42b:FITC.

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets with Mouse anti Human CD42b.

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets.

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets.

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD42b: RPE.

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - Analysis using the Biotin conjugate of NB100-63790. Staining of human peripheral blood platelets.

Immunoprecipitation: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Immunoprecipitation: CD42b/GPIb alpha Antibody (AK2) [NB100-63790] - After labeling the surface proteins of the CHO a beta IX cells with biotin, the GPIbIX complex was immunoprecipitated using the MoAb panel. The presence of the GPIba and GPIX native proteins was shown on the surface of the CHO a beta IX cells. Lane 1 shows the components immunoprecipitated using AK3, lane 2 with AK2, and lane 3 with SZ2. After cleavage with mocarhagin, AK3 precipitated a 70-kD GPIba component and the intact GPIX protein from the surface of the cells (lane 4). AK2 (lane 5) and SZ2 (lane 6) were able to precipitate a 40-kD GPIba component from the supernatant collected after the cleavage step.

View 4 more images

Applications for CD42b/GPIb alpha Antibody (AK2)

Application

Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:50-1:100

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD42b/GPIb alpha

CD42a is a small membrane glycoprotein found on the surface of human platelets. It forms a one to one noncovalent complex with glycoprotein Ib, a platelet surface membrane glycoprotein complex that functions as a receptor for von Willebrand factor. The main portion of the receptor is a heterodimer composed of 2 polypeptide chains, an alpha chain and a beta chain, that are linked by disulfide bonds. The complete receptor complex includes noncovalent association of the alpha and beta subunits with GP9 and platelet glycoprotein V. CD42a is present on thrombocytes and also on a so far unidentified subpopulation of T lymphocytes.

Long Name

Glycoprotein lb [Platelet] alpha

Alternate Names

BP1BA, BSS, CD42b, GP1BA, GPIb alpha

Gene Symbol

GP1BA

UniProt

P07359

Additional CD42b/GPIb alpha Products

Product Documents for CD42b/GPIb alpha Antibody (AK2)

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CD42b/GPIb alpha Antibody (AK2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

CD42b/GPIb alpha Antibody (AK2)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-63790

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Concentration

View all CD42b/GPIb alpha Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Specificity

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for CD42b/GPIb alpha Antibody (AK2)

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Flow Cytometry: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Immunoprecipitation: CD42b/GPIb alpha Antibody (AK2) - BSA Free [NB100-63790]

Applications for CD42b/GPIb alpha Antibody (AK2)

ELISA

Flow Cytometry

Immunoprecipitation

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: CD42b/GPIb alpha

Long Name

Alternate Names

Gene Symbol

UniProt

Additional CD42b/GPIb alpha Products

Product Documents for CD42b/GPIb alpha Antibody (AK2)

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CD42b/GPIb alpha Antibody (AK2)