CD43/Sialophorin Antibody (84-3C1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44649

Clone 84-3C1 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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NBP2-44649-0.1mg
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 84-3C1

Concentration

0.2 mg/ml

View all CD43 Antibodies »

Product Specifications

Immunogen

Stimulated human leukocytes

Localization

Cell surface

Specificity

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Marker

T-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11445)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD43/Sialophorin Antibody (84-3C1)

Western Blot: CD43/Sialophorin Antibody (84-3C1) [NBP2-44649]

Western Blot: CD43/Sialophorin Antibody (84-3C1) [NBP2-44649]

Western Blot: CD43/Sialophorin Antibody (84-3C1) [NBP2-44649] - Western Blot Analysis of K562 cell lysate using CD43/Sialophorin Antibody (84-3C1).
Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (84-3C1) [NBP2-44649]

Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (84-3C1) [NBP2-44649]

Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (84-3C1) [NBP2-44649] - Immunofluorescence Analysis of K562 cells labeling CD43 with CD43/Sialophorin Antibody (84-3C1) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot .
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (84-3C1) [NBP2-44649]

Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (84-3C1) [NBP2-44649]

Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (84-3C1) [NBP2-44649] - Human Tonsil stained with CD43 Monoclonal Antibody (84-3C1).
View 1 more images

Applications for CD43/Sialophorin Antibody (84-3C1)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD43

CD43 is a key glycoprotein of thymocytes and T lymphocytes, which affects the physicochemical properties of the T-cell surface and plays a role in lectin binding. This antibody contains an extended rodlike structure that may protrude above the glycocalyx of the cell, allowing accessibility of multiple glycan chains for binding. During T-cell activation CD43 is actively removed from the T-cell-antigen-presenting cell contact site, thus suggesting CD43's role in negative regulation of the adaptive immune response.

Long Name

Cluster of Differentiation 43

Alternate Names

CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN

Gene Symbol

SPN

UniProt

P16150

Additional CD43 Products

Product Documents for CD43/Sialophorin Antibody (84-3C1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD43/Sialophorin Antibody (84-3C1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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