CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12075

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Product Datasheet / COA

Recombinant Monoclonal Antibody

View Available Conjugates

Conjugate

Catalog #

Alexa Fluor 350

NBP3-12075AF350

Alexa Fluor 405

NBP3-12075AF405

Alexa Fluor 488

NBP3-12075AF488

Alexa Fluor 532

NBP3-12075AF532

Alexa Fluor 594

NBP3-12075AF594

Alexa Fluor 647

NBP3-12075AF647

Alexa Fluor 700

NBP3-12075AF700

Alexa Fluor 750

NBP3-12075AF750

Allophycocyanin

NBP3-12075APC

Biotin

NBP3-12075B

CoraFluor 1

NBP3-12075CL1

DyLight 350

NBP3-12075UV

DyLight 405

NBP3-12075V

DyLight 488

NBP3-12075G

DyLight 550

NBP3-12075R

DyLight 594

NBP3-12075DL594

DyLight 650

NBP3-12075C

DyLight 680

NBP3-12075FR

DyLight 755

NBP3-12075IR

FITC

NBP3-12075F

HRP

NBP3-12075H

Janelia Fluor 525

NBP3-12075JF525

Janelia Fluor 549

NBP3-12075JF549

Janelia Fluor 646

NBP3-12075JF646

Janelia Fluor 669

NBP3-12075JF669

mFluor Violet 450 SE

NBP3-12075MFV450

mFluor Violet 500 SE

NBP3-12075MFV500

mFluor Violet 610 SE

NBP3-12075MFV610

PE

NBP3-12075PE

PerCP

NBP3-12075PCP

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 84-3C1

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD43 Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunizng mice with stimulated human leukocytes.

Specificity

This antibody recognizes CD43/Sialophorin which is a predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free

Western Blot: CD43/Sialophorin Antibody (84-3C1)ChimericAzide and BSA Free [NBP3-12075]

Western Blot: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Daudi(A) cell lysate, K562(B) cell lysate and human bone marrow(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 84-3C1f (NBP3-12075) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free [NBP3-12075]

Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 84-3C1 (NBP3-12075) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12075, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD43

CD43 is a key glycoprotein of thymocytes and T lymphocytes, which affects the physicochemical properties of the T-cell surface and plays a role in lectin binding. This antibody contains an extended rodlike structure that may protrude above the glycocalyx of the cell, allowing accessibility of multiple glycan chains for binding. During T-cell activation CD43 is actively removed from the T-cell-antigen-presenting cell contact site, thus suggesting CD43's role in negative regulation of the adaptive immune response.

Long Name

Cluster of Differentiation 43

Alternate Names

CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN

Gene Symbol

SPN

Additional CD43 Products

Product Documents for CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free

Product Datasheets

Download Product Datasheets

COA

Product Specific Notices for CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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