CD43/Sialophorin Antibody (SPN/2049R) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08244

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SPN/2049R

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD43 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human CD43/Sialophorin protein (Uniprot: P16150)

Localization

Cell Surface

Specificity

It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. From 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Marker

T-Cell Marker

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

1.0 mg/ml of antibody purified by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07239).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD43/Sialophorin Antibody (SPN/2049R) - Azide and BSA Free

Western Blot: CD43/Sialophorin Antibody (SPN/2049R)Azide and BSA Free [NBP3-08244]

Western Blot: CD43/Sialophorin Antibody (SPN/2049R)Azide and BSA Free [NBP3-08244]

Western Blot: CD43/Sialophorin Antibody (SPN/2049R) - Azide and BSA Free [NBP3-08244] - Western Blot Analysis of K562 cell lysate using CD43/Sialophorin Rabbit Recombinant Monoclonal Antibody (SPN/2049R)
Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (SPN/2049R) - Azide and BSA Free [NBP3-08244]

Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (SPN/2049R) - Azide and BSA Free [NBP3-08244]

Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (SPN/2049R) - Azide and BSA Free [NBP3-08244] - Immunofluorescence Analysis of K562 cells labeling CD43/Sialophorin with CD43/Sialophorin Rabbit Recombinant Monoclonal Antibody (SPN/2049R) followed by Goat anti-rabbit IgG-CF488 (Green). The nuclear counterstain is NucSpot.
Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPN/2049R) - Azide and BSA Free [NBP3-08244]

Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPN/2049R) - Azide and BSA Free [NBP3-08244]

Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (SPN/2049R) - Azide and BSA Free [NBP3-08244] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD43/Sialophorin Rabbit Recombinant Monoclonal Antibody (SPN/2049R).
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Applications for CD43/Sialophorin Antibody (SPN/2049R) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD43

CD43 is a key glycoprotein of thymocytes and T lymphocytes, which affects the physicochemical properties of the T-cell surface and plays a role in lectin binding. This antibody contains an extended rodlike structure that may protrude above the glycocalyx of the cell, allowing accessibility of multiple glycan chains for binding. During T-cell activation CD43 is actively removed from the T-cell-antigen-presenting cell contact site, thus suggesting CD43's role in negative regulation of the adaptive immune response.

Long Name

Cluster of Differentiation 43

Alternate Names

CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN

Gene Symbol

SPN

Additional CD43 Products

Product Documents for CD43/Sialophorin Antibody (SPN/2049R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD43/Sialophorin Antibody (SPN/2049R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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