Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 5035-41.1D

Concentration

0.1 mg/ml

View all CD44 Antibodies »

Product Specifications

Immunogen

Spleen cells from B6PL-Ly-2a / Ly-3a B6-Ly-1a mice

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Human reactivity reported in scientific literature (PMID:32901011)

Specificity

This recognizes the following strains of mice:- C57BL/6, C57BL/10, C57/L, C58A, AKR, 129, SJL, NZB, C3H, CE, CBA/H.

Marker

Cell Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD44 Antibody (5035-41.1D) [FITC]

Flow Cytometry: CD44 Antibody (5035-41.1D) [FITC] [NB100-63801] - Staining of mouse spleen cells with MOUSE ANTI MOUSE CD44:FITC

Applications for CD44 Antibody (5035-41.1D) [FITC]

Application
Recommended Usage

Flow Cytometry

1:10-1:1000
Application Notes
For Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD44

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

12505 (Mouse)

Gene Symbol

CD44

UniProt

P15379 (Mouse)

Additional CD44 Products

Product Documents for CD44 Antibody (5035-41.1D) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD44 Antibody (5035-41.1D) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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