ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, SDS-Page, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 8E2F3

Format

BSA Free

Concentration

1.0 mg/ml

View all CD44 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CD44 (628-699) expressed in E. coli.

Reactivity Notes

Not reactive to rabbit per customer review.

Localization

Membrane; Single-pass type I membrane protein.

Marker

Cell Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD44 Antibody (8E2F3) - BSA Free

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of paraffin-embedded human lung cancer tissues using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).

Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of FFPE human breast carcinoma tissues using CD44 antibody (8E2F3). The signal was developed using DAB based detection and the sections were processed for counterstaining with hematoxylin. The antibody generated mainly a membrane staining representative of CD44 protein.

Flow Cytometry: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of HeLa cells using anti-CD44 mAb (right) and negative control (left).

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-CD44 Antibody [8E2F3] conjugated to DyLight 550 (NBP1-47386R) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Western Blot: CD44 Antibody (8E2F3)BSA Free [NBP1-47386]

Western Blot: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - Generation and characterization of immortalized primary keratinocytes (IPKs) from K14Cre.Ptpn2w/w and K14Cre.Ptpn2fl/fl mice. Western blot analysis of pSTAT3, STAT3, and CD44 (NBP1-47386) in primary keratinocytes and IPKs from both genotypes. The A431 cells were used as positive controls. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29955047/) licensed under a CC-BY license.

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD44 Antibody [8E2F3] conjugated to Alexa Fluor 647 (NBP1-47386AF647) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

Flow Cytometry: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Flow Cytometry: CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of methanol-fixed A431 (A), HeLa (B), PANC-1 (C) and EC (D) cells using anti-CD44 mAb (green), showing membrane localization. DRAQ5 fluorescent DNA dye (blue).

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Immunocytochemistry/Immunofluorescence: CD44 Antibody (8E2F3) [NBP1-47386] - Analysis of PANC-1 cells using anti-CD44 mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). DRAQ5 fluorescent DNA dye (blue).

Flow (Intracellular): CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Flow (Intracellular): CD44 Antibody (8E2F3) [NBP1-47386] - An intracellular stain was performed on HeLa cells with NBP1-47386AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Immunoprecipitation: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Immunoprecipitation: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386] - HAP1 lysates were prepared and immunoprecipitation was performed using 1.0 ug of the CD44 Antibody (NBP1-47386) pre-coupled to either protein G or protein A Sepharose beads. Ability of the antibodies to capture CD44 antigen was first assessed by comparing the level of CD44 antigen from the starting material (SM) to its level remaining in the unbound fractions (UB). Anti-CD44 antigen at 1/2000 was used for each immunoblot. Immunoprecipitate for CD44 Antibody (NBP1-47386) that showed depleted CD44 antigen in the UB can be seen. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

CD44 (8E2F3) in A431 Human Cell Line -

CD44 (8E2F3) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti- CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to Biotin (Catalog # NBP1-47386B) at 2 µg/mL overnight at 4C. Cells were stained using Streptavidin conjugated to DyLight 550 (red) and counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

CD44 (8E2F3) in A431 Human Cell Line -

CD44 (8E2F3) was detected in immersion fixed A431 human skin carcinoma cell line using Mouse anti-CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-47386R) (red) at 5 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

CD44 (8E2F3) in NIH-3T3 Mouse Cell Line.

CD44 (8E2F3) was detected in immersion fixed NIH3T3 Mouse fibroblast cell line using Mouse anti- CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-47386R) (red) at 2 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Detection of CD44 (8E2F3) in U-251 MG Human Cell Line by Flow Cytometry.

U-251 MG human glioblastoma cell line was stained with Mouse anti-CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-47386AF647, blue histogram) or matched control antibody (orange histogram).

Detection of CD44 (8E2F3) in A431 Human Cell Line by Flow Cytometry.

An intracellular stain was performed on A431 human skin carcinoma cell line using Mouse anti-CD44 (8E2F3) Protein-G purified Monoclonal Antibody conjugated to DyLight 550 (Catalog # NBP1-47386R, blue histogram) or matched control antibody (orange histogram) at 2.5 µg/mL for 30 minutes at RT.

View 13 more images

Applications for CD44 Antibody (8E2F3) - BSA Free

Application

Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-47386 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD44

CD44, also known as IN, LHRMIC4, CDW44, HCELL, is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

960 (Human)

Gene Symbol

CD44

UniProt

P16070

Additional CD44 Products

Product Documents for CD44 Antibody (8E2F3) - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for CD44 Antibody (8E2F3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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CD44 Antibody (8E2F3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47386

Key Product Details

Species Reactivity

Validated:

Cited:

Predicted:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all CD44 Antibodies »

Product Specifications

Immunogen

Reactivity Notes

Localization

Marker

Clonality

Host

Isotype

Theoretical MW

Scientific Data Images for CD44 Antibody (8E2F3) - BSA Free

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Immunohistochemistry-Paraffin: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Flow Cytometry: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Western Blot: CD44 Antibody (8E2F3)BSA Free [NBP1-47386]

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Flow Cytometry: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Flow (Intracellular): CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

Immunoprecipitation: CD44 Antibody (8E2F3) - BSA Free [NBP1-47386]

CD44 (8E2F3) in A431 Human Cell Line -

CD44 (8E2F3) in A431 Human Cell Line -

CD44 (8E2F3) in NIH-3T3 Mouse Cell Line.

Detection of CD44 (8E2F3) in U-251 MG Human Cell Line by Flow Cytometry.

Detection of CD44 (8E2F3) in A431 Human Cell Line by Flow Cytometry.

Applications for CD44 Antibody (8E2F3) - BSA Free

ELISA

Flow Cytometry

Immunocytochemistry/ Immunofluorescence

Immunohistochemistry

Immunohistochemistry-Paraffin

Reviewed Applications

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Shipping

Stability & Storage

Background: CD44

Alternate Names

Entrez Gene IDs

Gene Symbol

UniProt

Additional CD44 Products

Product Documents for CD44 Antibody (8E2F3) - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for CD44 Antibody (8E2F3) - BSA Free