CD44 Antibody (9A4) - variant 6

Novus Biologicals, part of Bio-Techne | Catalog # NB100-64818

Novus Biologicals, part of Bio-Techne
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NB100-64818
NB100-64818-0.025mg
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry, IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 9A4

Concentration

0.5 mg/ml

View all CD44 Antibodies »

Product Specifications

Immunogen

GST-CD44v6 fusion protein

Specificity

Recognizes an epitope encoded by exon v6 on the variant portion of murine CD44.

Marker

Cell membrane Marker

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for CD44 Antibody (9A4) - variant 6

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
For IHC-P: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Use in FLOW reported in scientific literature (PMID: 30504836).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

<0.1% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD44

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

12505 (Mouse)

Gene Symbol

CD44

UniProt

P15379 (Mouse)

Additional CD44 Products

Product Documents for CD44 Antibody (9A4) - variant 6

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD44 Antibody (9A4) - variant 6

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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