CD44 Antibody (9W6E7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15617

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15617-20ul
NBP3-15617-100ul
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9W6E7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD44 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-178 of human CD44 (NP_000601.3). AQIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD44 Antibody (9W6E7)

Western Blot: CD44 Antibody (9W6E7) [NBP3-15617]

Western Blot: CD44 Antibody (9W6E7) [NBP3-15617]

Western Blot: CD44 Antibody (2V9T2) [NBP3-15617] - Western blot analysis of extracts of U-251MG cells, using CD44 antibody (NBP3-15617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: CD44 Antibody (9W6E7) [NBP3-15617]

Immunohistochemistry-Paraffin: CD44 Antibody (9W6E7) [NBP3-15617]

Immunohistochemistry-Paraffin: CD44 Antibody (2V9T2) [NBP3-15617] - Immunohistochemistry of paraffin-embedded rat spleen using CD44 Rabbit mAb (NBP3-15617) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: CD44 Antibody (9W6E7) [NBP3-15617]

Immunohistochemistry-Paraffin: CD44 Antibody (9W6E7) [NBP3-15617]

Immunohistochemistry-Paraffin: CD44 Antibody (2V9T2) [NBP3-15617] - Immunohistochemistry of paraffin-embedded human esophageal using CD44 Rabbit mAb (NBP3-15617) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for CD44 Antibody (9W6E7)

Application
Recommended Usage

Flow Cytometry

0.2ug per 10^6 cells in 100 ul

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Western Blot

1:1000 - 1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD44

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

Additional CD44 Products

Product Documents for CD44 Antibody (9W6E7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD44 Antibody (9W6E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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