CD44 Antibody (DF1485) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11454

Novus Biologicals, part of Bio-Techne
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NBP3-11454
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DF1485

Format

Azide and BSA Free

Concentration

1 mg/ml

View all CD44 Antibodies »

Product Specifications

Immunogen

Purified CD44 antigen (PGp-1) from lymphocyte membrane (Uniprot: P16070)

Localization

Cell surface

Specificity

Recognizes a cell surface glycoprotein of 80-95kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44653).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD44 Antibody (DF1485) - Azide and BSA Free

CD44 Antibody (DF1485) - Azide and BSA Free Immunohistochemistry-Paraffin: CD44 Antibody (DF1485) - Azide and BSA Free [NBP3-11454] -

Immunohistochemistry-Paraffin: CD44 Antibody (DF1485) - Azide and BSA Free [NBP3-11454] -

Immunohistochemistry-Paraffin: CD44 Antibody (DF1485) - Azide and BSA Free [NBP3-11454] - Formalin-fixed, paraffin-embedded human Breast tissue stained with CD44 Antibody (DF1485).
CD44 Antibody (DF1485) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: CD44 Antibody (DF1485) - Azide and BSA Free [NBP3-11454] -

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (DF1485) - Azide and BSA Free [NBP3-11454] -

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (DF1485) - Azide and BSA Free [NBP3-11454] - Confocal immunofluorescence image HeLa cells using labeled Green (CF488) and Red Dot is used to label the nuclei Red.

Applications for CD44 Antibody (DF1485) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD44

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

Additional CD44 Products

Product Documents for CD44 Antibody (DF1485) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD44 Antibody (DF1485) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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