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CD44 Antibody (OX-50) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80649

Novus Biologicals, part of Bio-Techne
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NBP2-80649
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Key Product Details

Species Reactivity

Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-50

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Rat T cell blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleen cells.

Localization

Type I membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD44 Antibody (OX-50) - Azide and BSA Free

Immunocytochemistry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]

Immunocytochemistry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]

Immunocytochemistry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649] - Analysis of a FFPE tissue section of Rat small intestine using Mouse anti-Rat CD44 antibody (clone OX50) at 1:100 dilution. The staining was detected using HRP-conjugated anti-Mouse secondary antibody with DAB reagent followed by hematoxylin counterstaini
Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]

Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]

Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649] - Analysis using the FITC conjugate of NB600-1317. Staining of rat spleen cells with Mouse anti Rat CD44: FITC.
Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]

Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649]

Flow Cytometry: CD44 Antibody (OX-50) - Azide and BSA Free [NBP2-80649] - Rat Splenocytes were stained with CD44 (OX-50) antibody NB600-1317(blue) and a matched isotype control NBP2-27287 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.

Applications for CD44 Antibody (OX-50) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:50 - 1:100

Immunohistochemistry

1:50 - 1:100

Immunohistochemistry-Frozen

1:50 - 1:100. Use reported in scientific literature (PMID 16673425)

Immunohistochemistry-Paraffin

1:50 - 1:100. Use reported in scientific literature (PMID 16673425)

Western Blot

reported in scientific literature (PMID 8537452)
Application Notes
This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD44

CD44 is a glycoprotrein of 85 kDa, with expression being increased following activation. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

Additional CD44 Products

Product Documents for CD44 Antibody (OX-50) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD44 Antibody (OX-50) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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