CD44 Antibody (VFF-8) - variant 5

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65533

Novus Biologicals, part of Bio-Techne
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NB100-65533
NB100-65533-0.025mg
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # VFF-8

Concentration

0.1 mg/ml

View all CD44 Antibodies »

Product Specifications

Immunogen

Made to Human CD44v5

Specificity

Specifically recognizes an epitope encoded by exon v5 on the variant portion of human CD44.

Marker

Cell Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD44 Antibody (VFF-8) - variant 5

Immunohistochemistry-Paraffin: CD44 Antibody (VFF-8) - variant 5 [NB100-65533]

Immunohistochemistry-Paraffin: CD44 Antibody (VFF-8) - variant 5 [NB100-65533]

Immunohistochemistry-Paraffin: CD44, variant 5 Antibody (VFF-8) [NB100-65533] - Human breast cancer tissue with Mouse anti Human CD44v5 antibody.
Immunohistochemistry-Paraffin: CD44 Antibody (VFF-8) - variant 5 [NB100-65533]

Immunohistochemistry-Paraffin: CD44 Antibody (VFF-8) - variant 5 [NB100-65533]

Immunohistochemistry-Paraffin: CD44, variant 5 Antibody (VFF-8) [NB100-65533] - Human breast cancer tissue with Mouse anti Human CD44v5 antibody.

Applications for CD44 Antibody (VFF-8) - variant 5

Application
Recommended Usage

Flow Cytometry

1:10

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10

Immunohistochemistry-Paraffin

1:10

Western Blot

1:100-1:2000
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium Citrate buffer pH6.0 is recommended for this purpose.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

<0.1% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD44

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

960 (Human)

Gene Symbol

CD44

UniProt

P16070

Additional CD44 Products

Product Documents for CD44 Antibody (VFF-8) - variant 5

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD44 Antibody (VFF-8) - variant 5

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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