CD45 Antibody (5C16) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-93609

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2 Clone # 5C16

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all CD45 Antibodies »

Product Specifications

Immunogen

This CD45 Antibody (5C16) was prepared from Cell lysates of mouse leukocytes.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Theoretical MW

145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD45 Antibody (5C16) - Azide and BSA Free

Immunohistochemistry-Paraffin: CD45 Antibody (5C16) - Azide and BSA Free [NB110-93609]

Immunohistochemistry-Paraffin: CD45 Antibody (5C16) - Azide and BSA Free [NB110-93609]

Immunohistochemistry-Paraffin: CD45 Antibody (5C16) [NB110-93609] - Correlation of MAP17 expression with inflammation (CD45) in human samples of tumors of different originMAP17 and CD45 staining of serial sections of human samples of tumors of different origin. MAP17 staining in all samples showed high expression levels, correlated with the inflammatory marker CD45. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/21651) licensed under a CC-BY license.
Immunohistochemistry: CD45 Antibody (5C16) - Azide and BSA Free [NB110-93609]

Immunohistochemistry: CD45 Antibody (5C16) - Azide and BSA Free [NB110-93609]

Immunohistochemistry: CD45 Antibody (5C16) [NB110-93609] - CD45 antibody (5C16) [NB110-93609] immunohistochemistry staining of paraffin sections of mouse kidney and liver tissues from LPS exposed animals.
CD45 Antibody (5C16) - Azide and BSA Free

Immunohistochemistry: CD45 Antibody (5C16) - Azide and BSA Free [NB110-93609] -

Immunohistochemistry: CD45 Antibody (5C16) - Azide and BSA Free [NB110-93609] - Correlation of MAP17 expression with inflammation in human samples of Crohn's disease & psoriasisMAP17, CD45 & CD68 staining of histological slides of human samples of psoriasis & Crohn's disease. MAP17 staining in both inflammatory diseases showed high expression levels, correlated with the inflammatory markers CD45 & CD68. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29228712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CD45 Antibody (5C16) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:100-1:400

Immunoprecipitation

1:100

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute with sterilized PBS to a final concentration of 0.4 mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS. 0.025 mg size is provided in liquid form, PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CD45

CD45, also known as leukocyte common antigen (LCA), T200, or Ly5, is a member C of the class 1 receptor-like protein tyrosine phosphatase (PTPRC) family (1, 2). It is a transmembrane glycoprotein which, due to alternative splicing, has a multiple isoforms with a theoretical molecular weight ranging from 180 - 220 kDa (1, 3-5). Human CD45 is synthesized as a 1281 amino acid sequence consisting of an alternatively spliced extracellular receptor-like region, a cysteine-rich domain, fibronectin-like III repeats, a transmembrane segment, and a cytoplasmic region with tandem protein tyrosine phosphatase (PTP) domains: the membrane proximal domain (D1) and the membrane distal domain (D2) (3, 5). CD45 is expressed on all nucleated hematopoietic cells and their precursors, except mature red blood cells, and is one of the most abundantly-expressed cell-surface glycoproteins, comprising approximately 10% of surface proteins in lymphocytes (3). Functionally, CD45 is essential for development and activation of T-cells and B-cells (1-5). More specifically, CD45 positively regulates antigen receptor signaling and Src-family member kinase activity (1, 3). There are many ways to regulate CD45 phosphatase activity including ligand binding, dimerization, protein interactions, cellular localization, and covalent modifications (3, 6). Ligands for CD45 include pUL11, a transmembrane protein of the cytomegalovirus RL11 (CMV RL11) family, and placental protein 14 (PP14), both of which exclusively bind CD45, and various lectins including CD22, galectin-1, galectin-3, macrophage mannose receptor (MR), and macrophage galactose-type lectin (MGL) (6).

Given its role in immune cell development and activation, CD45 has also been linked to a variety of diseases. The importance of CD45 in immunity has been revealed in human and mouse studies where CD45-deficiency leads to a severe-combined immunodeficiency (SCID) phenotype (2, 3, 6). A CD45-knockout mice study revealed inhibited thymocyte production and poor B-cell response, whereas CD45 activation in mice causes lymphoproliferation and autoantibody production (3). CD45 variants have been associated with altered immune function and autoimmune disorders including multiple sclerosis, systemic lupus erythematosus (SLE), and rheumatoid arthritis (6). Furthermore, altered CD45 expression has been implicated in oncological conditions including chronic lymphatic leukemia, acute lymphatic leukemia, Hodgkin lymphoma, multiple myeloma, and diffuse large B-cell lymphoma (6). Considering its role in autoimmune disorders, immunodeficiency and cancer, CD45 is an ideal therapeutic target (3, 6). The main approaches to control CD45 function is through either selective inhibitors or anti-CD45 antibodies (3).

Alternative names for CD45 includes B220, CD antigen: CD45, CD45 antigen, CD45R, EC 3.1.3.48, GP180, LCA, Leukocyte common antigen, LY5, protein tyrosine phosphatase receptor type c polypeptide, PTPRC, receptor-type tyrosine-protein phosphatase C, T200 Glycoprotein, and T200.

References

1. Trowbridge, I. S., & Thomas, M. L. (1994). CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annual review of immunology. https://doi.org/10.1146/annurev.iy.12.040194.000505

2. Andersen, J. N., Jansen, P. G., Echwald, S. M., Mortensen, O. H., Fukada, T., Del Vecchio, R., Tonks, N. K., & Moller, N. P. (2004). A genomic perspective on protein tyrosine phosphatases: gene structure, pseudogenes, and genetic disease linkage. FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

3. Hermiston, M. L., Xu, Z., & Weiss, A. (2003). CD45: a critical regulator of signaling thresholds in immune cells. Annual review of immunology. https://doi.org/10.1146/annurev.immunol.21.120601.140946

4. Tonks, N. K., Diltz, C. D., & Fischer, E. H. (1990). CD45, an integral membrane protein tyrosine phosphatase. Characterization of enzyme activity. The Journal of biological chemistry.

5. Nam, H. J., Poy, F., Saito, H., & Frederick, C. A. (2005). Structural basis for the function and regulation of the receptor protein tyrosine phosphatase CD45. The Journal of experimental medicine. https://doi.org/10.1084/jem.20041890

6. Rheinlander, A., Schraven, B., & Bommhardt, U. (2018). CD45 in human physiology and clinical medicine. Immunology letters. https://doi.org/10.1016/j.imlet.2018.01.009

Long Name

Cluster of Differentiation 45

Alternate Names

CD45, LCA, PTPRC, T200 Glycoprotein

Entrez Gene IDs

19264 (Mouse)

Gene Symbol

PTPRC

UniProt

P06800 (Mouse)

Additional CD45 Products

Product Documents for CD45 Antibody (5C16) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD45 Antibody (5C16) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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