CD45 Antibody (YKIX716.13)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-65906

Novus Biologicals, part of Bio-Techne
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NB100-65906-0.025mg
NB100-65906-0.1mg
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Key Product Details

Species Reactivity

Validated:

Canine

Cited:

Equine

Applications

Validated:

Flow Cytometry, Immunoprecipitation

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # YKIX716.13

Concentration

1.0 mg/ml

View all CD45 Antibodies »

Product Specifications

Immunogen

Canine thymocytes

Reactivity Notes

CD45 Antibody (YKIX716.13) shows reactivity with Equine as reported in multiple pieces of scientific literature.

Specificity

CD45 Antibody (YKIX716.13) recognizes canine CD45 also known as leukocyte common antigen lustered as Canine CD45 in the First Canine Leukocyte Antigen Workshop. CD45 is expressed on all leukocytes in canine peripheral blood and may be against CD45RB isoform. This antibody reacts with CD45 on all outbred mongrels and beagles tested.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Theoretical MW

147 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.

Scientific Data Images for CD45 Antibody (YKIX716.13)

Flow Cytometry: CD45 Antibody (YKIX716.13) - BSA Free [NB100-65906]

Flow Cytometry: CD45 Antibody (YKIX716.13) - BSA Free [NB100-65906]

Flow Cytometry: CD45 Antibody (YKIX716.13) [NB100-65906] - Staining of canine peripheral blood lymphocytes with rat anti-canine CD45 antibody (YKIX716.13) [NB100-65906], using F(ab')2 rabbit anti rat IgG: FITC (STAR17B) as a secondary antibody.
CD45 Antibody (YKIX716.13)

Flow Cytometry: CD45 Antibody (YKIX716.13) [NB100-65906] -

Flow Cytometry: CD45 Antibody (YKIX716.13) [NB100-65906] - Immunophenotyping & multipotency assay. Representative dot plots from flow cytometry analysis (A). Expression of surface antigens were investigated in cells cultured in control & AZA/RES supplemented medium. Isolated cells were characterized by the expression of CD90 & CD44 while lacked the expression of CD45 hematopoietic marker (B). Multipotency of isolated cells was confirmed by three lineage differentiation (C). In order to confirm formation of proteoglycans during chondrogenesis cells were stained with Safranin O. Intracellular lipid droplets were visualized by Oil Red O while mineralized matrix formed during osteogenesis with Alizarin Red. Magnification ×100, scale bars: 250 μm. Results expressed as mean ± SD. Statistical significance indicated as asterisk (*) when comparing the result to ASCCTRL, & as hashtag (#) when comparing to ASCEMS. ###P < 0.001 Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30370650), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CD45 Antibody (YKIX716.13)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunoprecipitation

1:10-1:500

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD45

CD45, also known as leukocyte common antigen (LCA), T200, or Ly5, is a member C of the class 1 receptor-like protein tyrosine phosphatase (PTPRC) family (1, 2). It is a transmembrane glycoprotein which, due to alternative splicing, has a multiple isoforms with a theoretical molecular weight ranging from 180 - 220 kDa (1, 3-5). Human CD45 is synthesized as a 1281 amino acid sequence consisting of an alternatively spliced extracellular receptor-like region, a cysteine-rich domain, fibronectin-like III repeats, a transmembrane segment, and a cytoplasmic region with tandem protein tyrosine phosphatase (PTP) domains: the membrane proximal domain (D1) and the membrane distal domain (D2) (3, 5). CD45 is expressed on all nucleated hematopoietic cells and their precursors, except mature red blood cells, and is one of the most abundantly-expressed cell-surface glycoproteins, comprising approximately 10% of surface proteins in lymphocytes (3). Functionally, CD45 is essential for development and activation of T-cells and B-cells (1-5). More specifically, CD45 positively regulates antigen receptor signaling and Src-family member kinase activity (1, 3). There are many ways to regulate CD45 phosphatase activity including ligand binding, dimerization, protein interactions, cellular localization, and covalent modifications (3, 6). Ligands for CD45 include pUL11, a transmembrane protein of the cytomegalovirus RL11 (CMV RL11) family, and placental protein 14 (PP14), both of which exclusively bind CD45, and various lectins including CD22, galectin-1, galectin-3, macrophage mannose receptor (MR), and macrophage galactose-type lectin (MGL) (6).

Given its role in immune cell development and activation, CD45 has also been linked to a variety of diseases. The importance of CD45 in immunity has been revealed in human and mouse studies where CD45-deficiency leads to a severe-combined immunodeficiency (SCID) phenotype (2, 3, 6). A CD45-knockout mice study revealed inhibited thymocyte production and poor B-cell response, whereas CD45 activation in mice causes lymphoproliferation and autoantibody production (3). CD45 variants have been associated with altered immune function and autoimmune disorders including multiple sclerosis, systemic lupus erythematosus (SLE), and rheumatoid arthritis (6). Furthermore, altered CD45 expression has been implicated in oncological conditions including chronic lymphatic leukemia, acute lymphatic leukemia, Hodgkin lymphoma, multiple myeloma, and diffuse large B-cell lymphoma (6). Considering its role in autoimmune disorders, immunodeficiency and cancer, CD45 is an ideal therapeutic target (3, 6). The main approaches to control CD45 function is through either selective inhibitors or anti-CD45 antibodies (3).

Alternative names for CD45 includes B220, CD antigen: CD45, CD45 antigen, CD45R, EC 3.1.3.48, GP180, LCA, Leukocyte common antigen, LY5, protein tyrosine phosphatase receptor type c polypeptide, PTPRC, receptor-type tyrosine-protein phosphatase C, T200 Glycoprotein, and T200.

References

1. Trowbridge, I. S., & Thomas, M. L. (1994). CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annual review of immunology. https://doi.org/10.1146/annurev.iy.12.040194.000505

2. Andersen, J. N., Jansen, P. G., Echwald, S. M., Mortensen, O. H., Fukada, T., Del Vecchio, R., Tonks, N. K., & Moller, N. P. (2004). A genomic perspective on protein tyrosine phosphatases: gene structure, pseudogenes, and genetic disease linkage. FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

3. Hermiston, M. L., Xu, Z., & Weiss, A. (2003). CD45: a critical regulator of signaling thresholds in immune cells. Annual review of immunology. https://doi.org/10.1146/annurev.immunol.21.120601.140946

4. Tonks, N. K., Diltz, C. D., & Fischer, E. H. (1990). CD45, an integral membrane protein tyrosine phosphatase. Characterization of enzyme activity. The Journal of biological chemistry.

5. Nam, H. J., Poy, F., Saito, H., & Frederick, C. A. (2005). Structural basis for the function and regulation of the receptor protein tyrosine phosphatase CD45. The Journal of experimental medicine. https://doi.org/10.1084/jem.20041890

6. Rheinlander, A., Schraven, B., & Bommhardt, U. (2018). CD45 in human physiology and clinical medicine. Immunology letters. https://doi.org/10.1016/j.imlet.2018.01.009

Long Name

Cluster of Differentiation 45

Alternate Names

CD45, LCA, PTPRC, T200 Glycoprotein

Gene Symbol

PTPRC

Additional CD45 Products

Product Documents for CD45 Antibody (YKIX716.13)

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Product Specific Notices for CD45 Antibody (YKIX716.13)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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