CD45RA Antibody (MEM-56) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-329

Clone MEM-56 was used by HLDA to establish CD designation.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # MEM-56

Format

BSA Free

Concentration

1.0 mg/ml

View all CD45RA Antibodies »

Product Specifications

Immunogen

Human thymocytes and T lymphocytes.

Specificity

The antibody MEM-56 reacts with CD45RA, a 205-220 kDa single chain type I glycoprotein, variant of CD45 (CD45RA isoform). CD45RA is expressed on most of B lymphocytes, resting and native T lymphocytes, medullar thymocytes and monocytes. HLDA IV; WS Code NL 907

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD45RA Antibody (MEM-56) - BSA Free

Western Blot: CD45RA Antibody (MEM-56)BSA Free [NB500-329]

Western Blot: CD45RA Antibody (MEM-56)BSA Free [NB500-329]

Western Blot: CD45RA Antibody (MEM-56) [NB500-329] - Fig. 1. Western Blotting analysis of whole cell lysate of HUT-78 human cutaneous T cell lymphoma cell line. Lane 1: immunostaining with anti-human CD4 degrees Control antibody Lane 2: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under non-reducing conditions Lane 3: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under reducing conditions.
Flow Cytometry: CD45RA Antibody (MEM-56) - BSA Free [NB500-329]

Flow Cytometry: CD45RA Antibody (MEM-56) - BSA Free [NB500-329]

Flow Cytometry: CD45RA Antibody (MEM-56) [NB500-329] - Staining of human peripheral blood with anti-CD45RA (MEM-56) PE.
Flow Cytometry: CD45RA Antibody (MEM-56) - BSA Free [NB500-329]

Flow Cytometry: CD45RA Antibody (MEM-56) - BSA Free [NB500-329]

Flow Cytometry: CD45RA Antibody (MEM-56) [NB500-329] - Surface staining (mass cytometry) of human peripheral blood with anti-CD45RA (MEM-56) 163Dy. Gated on singlets.
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Applications for CD45RA Antibody (MEM-56) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.3-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
Immunohistochemistry-Paraffin sections: No pre-treatment of tissue sections is essential. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD45RA

CD45RA is a high molecular weight isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on
cell type and physiological state of the cell. CD45RA is expressed e.g. on nave T cells and normal plasma cells.

Long Name

CD45R, A isoform

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Entrez Gene IDs

5788 (Human)

Gene Symbol

PTPRC

Additional CD45RA Products

Product Documents for CD45RA Antibody (MEM-56) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD45RA Antibody (MEM-56) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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