Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Rat

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OX-33

Concentration

1.0 mg/ml

View all CD45RA Antibodies »

Product Specifications

Immunogen

Purified Rat spleen L-CA

Localization

Membrane; single pass type I membrane protein.

Specificity

NB100-65000 is directed against a high molecular weight band of the leucocyte common antigen (LCA). The MRC OX-33 only labels B-cells among thoracic duct lymphocytes with little labelling in bone marrow and none on thymocytes (2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD45RA Antibody (OX-33) - (B Cells only)

Flow Cytometry: CD45RA Antibody (OX-33) - BSA Free [NB100-65000]

Flow Cytometry: CD45RA Antibody (OX-33) - BSA Free [NB100-65000]

Flow Cytometry: CD45RA Antibody (OX-33) [NB100-65000] - Staining of rat spleen cells with Mouse anti Rat CD45RA.
Flow Cytometry: CD45RA Antibody (OX-33) - BSA Free [NB100-65000]

Flow Cytometry: CD45RA Antibody (OX-33) - BSA Free [NB100-65000]

Flow Cytometry: CD45RA Antibody (OX-33) [NB100-65000] - Rat spleen cells.

Applications for CD45RA Antibody (OX-33) - (B Cells only)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
Membrane permeabilisation is required for Immunohistochemistry-Paraffin

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD45RA

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.

Long Name

CD45R, A isoform

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbol

PTPRC

UniProt

P04157 (Rat)

Additional CD45RA Products

Product Documents for CD45RA Antibody (OX-33) - (B Cells only)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD45RA Antibody (OX-33) - (B Cells only)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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