CD45RA Antibody (PTPRC/818)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44844

Novus Biologicals, part of Bio-Techne
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NBP2-44844-0.1mg
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # PTPRC/818

Concentration

0.2 mg/ml

View all CD45RA Antibodies »

Product Specifications

Immunogen

Stimulated human leukocytes

Localization

Cell surface

Specificity

Recognizes a protein of 205kDa-220kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This monoclonal antibody is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).

Marker

Leucocyte Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47957)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD45RA Antibody (PTPRC/818)

Western Blot: CD45RA Antibody (PTPRC/818) [NBP2-44844]

Western Blot: CD45RA Antibody (PTPRC/818) [NBP2-44844]

Western Blot: CD45RA Antibody (PTPRC/818) [NBP2-44844] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Antibody (PTPRC/818).
Immunocytochemistry/ Immunofluorescence: CD45RA Antibody (PTPRC/818) [NBP2-44844]

Immunocytochemistry/ Immunofluorescence: CD45RA Antibody (PTPRC/818) [NBP2-44844]

Immunocytochemistry/Immunofluorescence: CD45RA Antibody (PTPRC/818) [NBP2-44844] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeling CD45RA with CD45RA Antibody (PTPRC/818) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/818) [NBP2-44844]

Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/818) [NBP2-44844]

Immunohistochemistry-Paraffin: CD45RA Antibody (PTPRC/818) [NBP2-44844] - Human Tonsil stained with CD45RA Monoclonal Antibody (PTPRC/818).
View 1 more images

Applications for CD45RA Antibody (PTPRC/818)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD45RA

The CD45 molecule is comprised of different glycoproteins ranging from 180-240 kD. Expression of CD45 is found on all hemopoietic cells. Detection of the different isoforms can distinguish between different cell forms i.e. naive T cells and memory T cells. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. The functional activity of each CD45 isotype also varies.

Long Name

CD45R, A isoform

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbol

PTPRC

UniProt

P08575

Additional CD45RA Products

Product Documents for CD45RA Antibody (PTPRC/818)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for CD45RA Antibody (PTPRC/818)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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