CD45RB Antibody (DF-B1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44843
![Immunohistochemistry-Paraffin: CD45RB Antibody (DF-B1) [NBP2-44843]](https://resources.bio-techne.com/images/products/CD45RB-Antibody-DF-B1-Immunohistochemistry-Paraffin-NBP2-44843-img0001.jpg "Immunohistochemistry-Paraffin: CD45RB Antibody (DF-B1) [NBP2-44843]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat (Negative)
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Kappa Clone # DF-B1
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Extract from Alexander's hepatoma cell line L428 (Workshop V)
Reactivity Notes
Does not react with Rat.
Localization
Cell surface and cytoplasmic
Marker
B-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11447)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD45RB Antibody (DF-B1)
Immunohistochemistry-Paraffin: CD45RB Antibody (DF-B1) [NBP2-44843]
Immunohistochemistry-Paraffin: CD45RB Antibody (DF-B1) [NBP2-44843] - Human Tonsil stained with CD45RB Monoclonal Antibody (DF-B1).Applications for CD45RB Antibody (DF-B1)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD45RB
Long Name
CD45R, B isoform
Alternate Names
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbol
PTPRC
UniProt
Additional CD45RB Products
Product Documents for CD45RB Antibody (DF-B1)
Product Specific Notices for CD45RB Antibody (DF-B1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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