CD45RO Antibody (190-2F2.5) [PE/Cy5.5]
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47956PECY55
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence
Label
PE/Cy5.5 (Excitation = 488 nm, Emission = 695 nm)
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 190-2F2.5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Lentillectin Purified glyco proteins obtained from HuPBL and from a CLL (B cell).
Localization
Cell Surface
Specificity
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Marker
T-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Applications for CD45RO Antibody (190-2F2.5) [PE/Cy5.5]
Application
Recommended Usage
CyTOF-ready
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: CD45RO
Long Name
CD45R, O isoform
Alternate Names
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbol
PTPRC
Additional CD45RO Products
Product Documents for CD45RO Antibody (190-2F2.5) [PE/Cy5.5]
Product Specific Notices for CD45RO Antibody (190-2F2.5) [PE/Cy5.5]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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