CD45RO Antibody (SPM125) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34735
![Immunohistochemistry-Paraffin: CD45RO Antibody (SPM125) - Azide and BSA Free [NBP2-34735]](https://resources.bio-techne.com/images/products/CD45RO-Antibody-SPM125-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34735-img0001.jpg "Immunohistochemistry-Paraffin: CD45RO Antibody (SPM125) - Azide and BSA Free [NBP2-34735]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Chimpanzee, Monkey
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # SPM125
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Cultured human T-cells from an IL-2-dependent T-cell line (CA1)
Reactivity Notes
Common Marmoset
Localization
Cell surface
Specificity
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Marker
T-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32939).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD45RO Antibody (SPM125) - Azide and BSA Free
Immunohistochemistry-Paraffin: CD45RO Antibody (SPM125) - Azide and BSA Free [NBP2-34735]
Immunohistochemistry-Paraffin: CD45RO Antibody (SPM125) - Azide and BSA Free [NBP2-34735] - Formalin-fixed. paraffin-embedded human Lymphoma stained with CD45RO Monoclonal Antibody SPM125Applications for CD45RO Antibody (SPM125) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD45RO
Long Name
CD45R, O isoform
Alternate Names
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbol
PTPRC
Additional CD45RO Products
Product Documents for CD45RO Antibody (SPM125) - Azide and BSA Free
Product Specific Notices for CD45RO Antibody (SPM125) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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