CD45RO Antibody (T200/797) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-54315
![Immunohistochemistry-Paraffin: CD45RO Antibody (T200/797) - Azide and BSA Free [NBP2-54315]](https://resources.bio-techne.com/images/products/CD45RO-Antibody-T200-797-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54315-img0001.jpg "Immunohistochemistry-Paraffin: CD45RO Antibody (T200/797) - Azide and BSA Free [NBP2-54315]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # T200/797
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Human T-lymphocytes (Uniprot: P08575)
Localization
Cell surface
Specificity
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Marker
T-Cell Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
182 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44860).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD45RO Antibody (T200/797) - Azide and BSA Free
Immunohistochemistry-Paraffin: CD45RO Antibody (T200/797) - Azide and BSA Free [NBP2-54315]
Immunohistochemistry-Paraffin: CD45RO Antibody (T200/797) - Azide and BSA Free [NBP2-54315] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RO Monoclonal Antibody (T200/797).![Flow Cytometry: CD45RO Antibody (T200/797) - Azide and BSA Free [NBP2-54315]](https://resources.bio-techne.com/images/products/CD45RO-Antibody-T200-797-Azide-and-BSA-Free-Flow-Cytometry-NBP2-54315-img0002.jpg "Flow Cytometry: CD45RO Antibody (T200/797) - Azide and BSA Free [NBP2-54315]")
Flow Cytometry: CD45RO Antibody (T200/797) - Azide and BSA Free [NBP2-54315]
Flow Cytometry: CD45RO Antibody (T200/797) - Azide and BSA Free [NBP2-54315] - Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45RO Antibody (T200/797) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).Applications for CD45RO Antibody (T200/797) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells
Immunocytochemistry/ Immunofluorescence
0.5 - 1 ug/ml
Immunohistochemistry-Paraffin
0.25 - 0.5 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: CD45RO
Long Name
CD45R, O isoform
Alternate Names
B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide
Gene Symbol
PTPRC
Additional CD45RO Products
Product Documents for CD45RO Antibody (T200/797) - Azide and BSA Free
Product Specific Notices for CD45RO Antibody (T200/797) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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