Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa/IgG2a Kappa Clone # UCHL-1 + T200/797

Concentration

0.2 mg/ml

View all CD45RO Antibodies »

Product Specifications

Immunogen

Cultured human T-cells from an IL-2-dependent T-cell line (CA1) (UCHL-1); Human T-lymphocytes (T200/797)

Localization

Cell surface

Specificity

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Marker

T-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa/IgG2a Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54314)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD45RO Antibody (UCHL-1 + T200/797)

Western Blot: CD45RO Antibody (UCHL-1 + T200/797) [NBP2-44849]

Western Blot: CD45RO Antibody (UCHL-1 + T200/797) [NBP2-44849]

Western Blot: CD45RO Antibody (UCHL-1 + T200/797) [NBP2-44849] - Western Blot Analysis of human spleen tissue lysates using CD45RO Antibody (UCHL-1 + T200/797).
Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1 + T200/797) [NBP2-44849]

Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1 + T200/797) [NBP2-44849]

Immunohistochemistry-Paraffin: CD45RO Antibody (UCHL-1 + T200/797) [NBP2-44849] - Human Tonsil stained with CD45RO Monoclonal Antibody (UCHL-1 + T200/797).

Applications for CD45RO Antibody (UCHL-1 + T200/797)

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.25-0.5 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD45RO

CD45RO is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA). It is expressed on most thymocytes, about 45% of peripheral blood T cells, virtually all T cells in skin reactive infiltrates, and the majority of T cell malignancies. It is also found on a subset of B cells and on exceptional B cell lymphomas.

Long Name

CD45R, O isoform

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbol

PTPRC

UniProt

P08575

Additional CD45RO Products

Product Documents for CD45RO Antibody (UCHL-1 + T200/797)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for CD45RO Antibody (UCHL-1 + T200/797)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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