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CD45RO Antibody (UCHL-1) [Alexa Fluor® 532]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33104AF532

Novus Biologicals, part of Bio-Techne
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NBP2-33104AF532

Key Product Details

Species Reactivity

Human, Chimpanzee, Monkey

Applications

CyTOF-ready, Dual RNAscope ISH-IHC, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Alexa Fluor 532

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # UCHL-1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Cultured human T-cells from an IL-2-dependent T-cell line (CA1)

Reactivity Notes

Common Marmoset

Localization

Cell surface

Specificity

Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.

Marker

T-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for CD45RO Antibody (UCHL-1) [Alexa Fluor® 532]

CD45RO Antibody (UCHL-1) [Alexa Fluor® 532]

CD45RO Antibody (UCHL-1) [Alexa Fluor® 532] [NBP2-33104AF532] -

CD45RO Antibody (UCHL-1) [Alexa Fluor® 532] [NBP2-33104AF532] - Vial of Alexa Fluor 532 conjugated antibody. Alexa Fluor 532 is optimally excited at 532 nm by the Yellow-Green laser (561 nm) and has an emission maximum of 554 nm.

Applications for CD45RO Antibody (UCHL-1) [Alexa Fluor® 532]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Dual RNAscope ISH-IHC

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD45RO

CD45RO is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA). It is expressed on most thymocytes, about 45% of peripheral blood T cells, virtually all T cells in skin reactive infiltrates, and the majority of T cell malignancies. It is also found on a subset of B cells and on exceptional B cell lymphomas.

Long Name

CD45R, O isoform

Alternate Names

B220, CD45 antigen, CD45R, EC 3.1.3.48, L-CA, LY5, protein tyrosine phosphatase, receptor type, C, receptor-type tyrosine-protein phosphatase C, T200 glycoprotein, T200 leukocyte common antigen, T200receptor type, c polypeptide

Gene Symbol

PTPRC

Additional CD45RO Products

Product Documents for CD45RO Antibody (UCHL-1) [Alexa Fluor® 532]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CD45RO Antibody (UCHL-1) [Alexa Fluor® 532]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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