CD46 Antibody (001)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-90080

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP2-90080-50ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 001

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all CD46 Antibodies »

Product Summary for CD46 Antibody (001)

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD46 (Accession#: EAW93471.1; Met1-Asp328).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD46 Antibody (001)

Immunocytochemistry/ Immunofluorescence: CD46 Antibody (001) [NBP2-90080]

Immunocytochemistry/ Immunofluorescence: CD46 Antibody (001) [NBP2-90080]

Immunocytochemistry/Immunofluorescence: CD46 Antibody (001) [NBP2-90080] - Staining of CD46 in HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CD46 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: CD46 Antibody (001) [NBP2-90080]

Immunohistochemistry-Paraffin: CD46 Antibody (001) [NBP2-90080]

Immunohistochemistry-Paraffin: CD46 Antibody (001) [NBP2-90080] - Staining of human CD46 in human placenta with rabbit monoclonal antibody (1:200). Positive staining was localized to membrane of trophoblast.

Applications for CD46 Antibody (001)

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry-Paraffin

1:100-1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD46

CD46 is one of the membrane complement regulatory proteins. CD46 possesses C3b-binding and factor I cofactor activities which play important roles in regulation of complement activation pathway. CD46 is widely distributed on blood cells, endothelial cells, epithelial cells and tumor cell lines. CD46 exists as many isoforms in a variety of tissues. The antigen has a broad distribution and is present on leukocytes, platelets, endothelial cells, epithelial cells and fibroblasts. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. It is expressed on thymocytes, B cells, monocytes, granulocytes, NK cells, platelets, epithelial and endothelial cells.

Alternate Names

CD46, MCP

Gene Symbol

CD46

Additional CD46 Products

Product Documents for CD46 Antibody (001)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for CD46 Antibody (001)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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